The R-group ofasparagine, anamide derivativeof aspartic acid, has no acidic or basic properties, but it is polar and participates inhydrogen bondformation. It is hydrolyzed to aspartic acid and ammonia by the enzymeasparaginase. Inglycoproteins, the carbohydrate side chain is often linked through ...
We also identified the deamidation or isomerization site of the lysozyme samples after incubating them under acidic or basic conditions.KameokaGraduate School of Pharmaceutical SciencesDaisukeGraduate School of Pharmaceutical SciencesUedaGraduate School of Pharmaceutical SciencesTadashi...
Relative expression values are normalized to control gene (60S acidic ribosomal protein P0). qPCR was performed with the following primers: ASNS Fwd: 50-CAGAAGATGGATTTTTGGCTG-30 ASNS Rev: 50-TGTCCAGGAAGAAAAGGCTC-30 PHGDH Fwd: 50-GCAAAGAGGAGCTGATAGCG-30 PHGDH Rev: 50-TTCTCAGCTGCGTTGA...
Relative expression values are normalized to control gene (60S acidic ribosomal protein P0). qPCR was performed with the following primers: ASNS Fwd: 5′-CAGAAGATGGATTTTTGGCTG-3′ ASNS Rev: 5′-TGTCCAGGAAGAAAAGGCTC-3′ PHGDH Fwd: 5′-GCAAAGAGGAGCTGATAGCG-3′ PHGDH Rev: 5′-TTCTCAGC...
Deamidation rate at 40°C was faster in acidic buffers than in basic buffers. However, this trend is reversed at 5°C, attributed to the change in hydroxide ion concentration influenced by buffer and temperature. The apparent activation energy was higher for rates generated in an acidic buffer ...
The incorporated radiolabel was stable over long periods of time and at acidic or basic pH as long as elevated temperatures were avoided. The radiolabel was removed by treatment of the protein at pH 10 and 50 degrees C or with 0.2 M hydroxylamine at 50 degrees C for at least 30 min. ...
The main p31 isoform and the splicing variant p41 form of the Ii chain were digested to produce a discrete pattern of bands at mildly acidic pH (e.g., Figure 1A, track 2) whereas the α and β chains of MHC class II were comparatively resistant. We then used various protease inhibitors...
Why would they be considered nonpolar, polar, acidic, or basic? What is an essential amino acid? What is the name of the enzyme that attaches amino acid to the tRNA? Draw a structural formula of simple amino acid, and identify the carboxyl group...
the plant PNGases characterized to date have an optimum pH in the acidic region and show strong activity towards glycopeptides but weak activity towards denatured glycoproteins, suggesting that the plant PNGases work in acidic environments such as the vacuole or cell wall.50–59As for plant cytoso...
Although it shows a similar folding pattern to the propeptides of other papain family members[31,32]the cathepsin B pro region is relatively short. The isolated propeptide is a potent, selective, cathepsin B inhibitor[33]but the dissociation constant increases dramatically under acidic conditions. ...