The effect of L-asparaginase on various acute human lymphoblastic leukemia cell populations was evaluated in an investigation of its mechanism of action with respect to the blast cell cycle and the growth of the leukemic cell population. Immediately following the injection of the drug, the number ...
Topics discussed include its use for the treatment of acute lymphoblastic leukemia (ALL) and lymphoblastic lymphoma (LBL), its dosage and administration, its contradications, and its interactions and mechanism of action. Also noted are advice to patients on asparaginase use, and its prepar...
Mechanism of Action The mechanism of action of Elspar is thought to be based on selective killing of leukemic cells due to depletion of plasma asparagine. Some leukemic cells are unable to synthesize asparagine due to a lack of asparagine synthetase and are dependent on an exogenous source of ...
Fig. 2.The action mechanism of asparaginase in reducing tumor cell growth. 3.1Therapeutic applications ofl-asparaginase l-asparaginase enzyme has varied applications and is a single molecule for the treatment of multiple diseases. This enzyme has been long known for its chemotherapeutic application (t...
This section discusses the biochemical properties of L-Asparaginase, including its structure, catalytic mechanism, and factors influencing its activity and stability. Mechanisms of Action L-Asparaginase exerts its anti-cancer effects by depleting circulating asparagine, an essential amino acid for cancer ...
Experimental material on structurally and functional organization, regulation of biosynthesis and activity, mechanism of action, genetic determinants, heterologous expression of bacterial L-asparaginases is accumulated. The modern approaches to isolation and purification of these enzymes, some questions of prac...
Hyperthermophilic asparaginase mutants with enhanced substrate affinity and antineoplastic activity: structural insights on their mechanism of action. FASEB J. 2012;26(3):1161–71. https://doi.org/10.1096/fj.11-191254. Article CAS PubMed Google Scholar Beckett A, Gervais D. What makes a good ...
Despite the unique mechanism of action of this cytotoxic substance which shows relative selectivity with regard to the metabolism of malignant cells, some patients experience toxic effects during asparaginase therapy. Immunological reactions toward the foreign protein include enzyme inactivation without any ...
Central thrombosis or intracranial haem- orrhage as well as peripheral thrombosis and haemorrhage have been reported after asparaginase therapy.1-4 Although the precise mechanism for this effect remains unclear, asparaginase appears to deplete certain clotting factors as well as antithrombin III, ...
Hence, it will be interesting to test whether in the resistant KHYG1 cell line, mutated p53 (at codon 248) could positively modulate the expression of AsnS, repre- senting another mechanism of ASNase resistance. We demonstrated that ASNase-induced cell death was mediated by apoptosis, in ...