a CAR in NK cells, which afforded enhanced anti-tumor NK cell recognition, potentially enabling the next generation of allogeneic cell-based therapies in oncology. AsCas12a Ultra is an advanced CRISPR nuclease with significant advantages in basic research and in the production of gene edited cell ...
Author Correction: AsCas12a ultra nuclease facilitates the rapid generation of therapeutic cell medicinesBacterial geneticsCRISPR-Cas9 genome editingGenetic engineeringTargeted gene repairThese authors contributed equally: Liyang Zhang, John A. Zuris. The original version of this Article incorrectly listed ...
原代T细胞中,以RNP转染的AsCas12a Ultra能以93%的效率同时敲除3种基因。而利用AAV6作为同源重组模板,AsCas12a Ultra的基因敲入效率接近60%,同时敲入两种基因的效率也接近40%。NK细胞中,AsCas12a Ultra的基因敲除和基因敲入能力同样强大。 总体而言,本研究开发的AsCas12a Ultra具备强大的基因编辑能力:其在多种有临床...
Advancing the genetic engineering toolbox by combiningAsCas12aknock-in mice with ultra-compact screeningdoi:10.1038/s41467-025-56282-2Biological SciencesGeneticsCas12a is a next-generation geneediting tool that enables multiplexed gene targeting. Here, we present a mouse model that constitutively ...