(HDR), where single-stranded DNA oligonucleotide (ssODNs) are preferentially used as donor templates. We therefore tested the performance of AsCas12a Ultra on ssODN-mediated precise editing in human Jurkat cells, which are notorious for poor HDR rates32. Interestingly, titration of AsCas12a RNP ...
除基因敲除效率有明显提升外,AsCas12a Ultra还显著增强同源重组(HDR)介导的基因敲入效率:以单链DNA(ssDNA)为模板时, AsCas12a Ultra介导的HDR编辑效率较野生型提高了一倍(8% vs 16%)。研究还指出,AsCas12a Ultra在很多位点的HDR效率甚至已经超越了SpCas9。 目前的临床治疗研究中,CAR-T为代表的细胞改造是热门领域。