The purpose of our study was to elucidate the role of the histone methyltransferase enhancer of zeste homologue 2 (EZH2) in the pathophysiology of osteoarthritis (OA) and to develop a strategy to modulate EZH2 activity for OA treatment. The expression of
AGS cells were seeded in 24-well plates at a density of 6 × 104 cells per well in RPMI-1640 culture medium supplemented with 10% FCS. The firefly luciferase plasmid containing five copies of an NF-κB response element (Promega, Madison, WI, USA) was mixed with Renilla Luciferase plasmid...
we also measured the effect of SCF on cell cycle phase distribution in the pre-sorted CD117 cells. We found that there was a 9% increase in the per cent of cells in S-phase at 48 h after SCF treatment as compared with no SCF treatment (Supplementary Fig. 13). Hence, some componen...