AGS cells were seeded in 24-well plates at a density of 6 × 104 cells per well in RPMI-1640 culture medium supplemented with 10% FCS. The firefly luciferase plasmid containing five copies of an NF-κB response element (Promega, Madison, WI, USA) was mixed with Renilla Luciferase plasmid...
The purpose of our study was to elucidate the role of the histone methyltransferase enhancer of zeste homologue 2 (EZH2) in the pathophysiology of osteoarthritis (OA) and to develop a strategy to modulate EZH2 activity for OA treatment. The expression of
For experiments with CD117+, cells were isolated by magnetic separation and grown in cells, Iscove’s Modified Dulbecco's Media media containing insulin (10 ng ml−1), FCS (10%) and erythropoietin (1 U ml−1). Where indicated, 3 μg ml−1of SCF (recombinant murine ...