Additional file Additional file 1: Figure S1. TMED2, TMED3 and TMED4 expression in breast cancer cell lines. A: qRT-PCR detection of TEMD2, TMED3 and TMED4 in a plethora of breast cancer cell lines; B: qRT-PCR detection of TEMD2, TMED3 and TMED4 in MCF-7 and MDA-MB-231 cells...
c, Iron-biofortified VIT1 (containing an additional 40–50 µg/g iron) or IRT1 + FER1 (containing an additional 80–90 µg/g iron) cassava lines, showing the potential nutritional contribution toward the EARs for different demographic groups relative to baseline WT-TME 204 (10...
Abstract Main Results and discussion Conclusion and outlook Methods Data availability Code availability References Acknowledgements Funding Author information Ethics declarations Peer review Additional information Supplementary information Rights and permissions About this article AdvertisementNature...
Subsequently, the cytotoxicity of T-A-Ls to HK-2 cells was determined by using the CCK-8 kit in accordance with the manufacturer’s protocol. To explore the distribution of T-A-Ls in vitro, HK-2 cells were seeded in a confocal dish at a density of 1×104 for 24 h. FITC-labeled ...
To investigate the functional role of LINC00623 in PDAC, two PDAC cell lines (BxPC-3 and PANC-1) were used for in vitro and in vivo functional assays. LINC00623 expression was silenced using two short hairpin RNAs (shRNAs) and overexpressed using the dCAS9-SAM system (Additional file 13...
The first is related to additional stress and strain on people working in sales and marketing (Huang & Rust, Citation2018). The second risk is related to detachment from customers. Recent research shows a digital mismatch between organizations and customers. While a selling organization puts more ...
Additional information Competing interests The authors declare that they have no competing interests. Authors’ contributions SGN, SR and DS conceived of and designed the study, and carried out wet lab analysis and writing of the manuscript. MG carried out the fieldwork and contributed to the study...
To achieve our goal of a current estimate of annual worldwide UAPP, we carried out a systematic review of the scientific literature and additionally used publicly available mortality data from the WHO. This systematic review is based on a protocol (supplementS1) according to the Preferred Reportin...
Additional efficacy measures were the proportion of patients discontinuing the study because of inad- equate analgesia for any reason, patient-reported pain intensity scores on a 100-mm visual analog scale (VAS), and patient global assessments at 48 and 72 hours. Adverse effects were also ...
Additional information Competing interests The authors declare that they have no competing interests. Authors’ contributions SGN, SR and DS conceived of and designed the study, and carried out wet lab analysis and writing of the manuscript. MG carried out the fieldwork and contributed to the study...