array-basedCGH先天性心脏病染色体核型染色体异常基因组拷贝数变异目的应用高分辨芯片比较基因组杂交技术(aCGH),对先天性心脏病(先心病)患儿进行基因组拷贝数变异(CNVs)筛查,探讨染色体拷贝数异常与先心病的关系。方法对17例先心病患者进行染色体核型分析,再运用Agi-lent 8×60K DNA芯片筛查染色体拷贝数异常情况,之后...
Array-based CGH is a high-throughput technique to measure DNA copy number change across the genome. The DNA fragments or "clones" of test and reference samples are hybridized to mapped array fragments. Log2 intensity ratios of test to reference provide useful information about genome-wi...
Array-Based CGH for Genomic DNA Analysis - ULS Labeling 7 1 Before You Begin Procedural Notes Procedural Notes • To process SurePrint G3 CGH+SNP microarrays, refer to the Agilent Oligonucleotide Array-Based CGH for Genomic DNA Analysis (Enzymatic Labeling for Blood, Cells, or Tissues) ...
To this end, we designed a 12-plex CGH array (135k; 135 000 probes/subarray) (Roche Nimblegen) with exonic and intronic oligonucleotide probes covering 26 genes routinely analyzed in the laboratory. We tested control samples with known CNMs and patients for whom genetic causes underlying their...
SegmentStruct = cghcbs(CGHData) performs circular binary segmentation (CBS) on array-based comparative genomic hybridization (aCGH) data to determine the copy number alteration segments (neighboring regions of DNA that exhibit a statistical difference in copy number) and change points. Note The CBS...
Array-based comparative genomic hybridization (array-CGH) is a high throughput, high resolution technique for studying the genetics of cancer. Analysis of array-CGH data typically involves estimation of the underlying chromosome copy numbers from the log fluorescence ratios and segmenting the chromosome ...
4 Array-based CGH in a clinical genetics laboratory (constitutional) vs. the 2600 (1 Mb) chip was made by the referring clinician. The majority of microarray studies were initiated with the 606 chip; however, for most cases with ab- normal cytogenetics, the 1-Mb array was preferred ...
Interphase FISH, multiplex-FISH (M-FISH, SKY) and comparative genomic hybridisation (CGH) have emerged as the most significant of these. More recently, ... L Kearney,SW Horsley - 《Chromosoma》 被引量: 127发表: 2005年 Electronic light microscopy: present capabilities and future prospects Electro...
Quantitative mapping of amplicon structure by array CGH identifies CYP24 as a candidate oncogene Nat Genet, 25 (2000), pp. 144-146 View in ScopusGoogle Scholar [26] T Ebisawa, M Fukuchi, G Murakami, T Chiba, K Tanaka, T Imamura, K Miyazono Smurf1 interacts with transforming growth factor...
If necessary, we also used 'MCG X-tiling array' (X-array) containing 1001 BAC/PACs throughout X chromosome other than pseudoautosomal regions.12 The array-CGH analysis was performed as previously described.12,23 For several subjects we applied an oligonucleotide array (Agilent Human Genome CGH ...