chains the charge of arginine side-chains is relatively unaffected by the surrounding environment.1'1 As a consequence, arginine provides nature with a reliable means of placing a positive charge at any required position in a bio-molecule, such as in active sites or at protein-ligand interfaces...
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Arginine side-chains play a distinct role because of their high pKa and perpetual positive charge. An NMR method is presented, based on carbon-detected 13Cζ–15Nε correlation spectra, which allows probing the arginine side-chains and their dynamics at neutral-to-high pH. The methodology is ...
They hydrolyze the terminal guanidinium moiety of peptidyl Arg into peptidyl citrulline residue, altering the surface charges on the protein surface. PADs have a wide range of tissue distribution and play a role in many physiological processes. PAD2 and PAD4 are in general associated with neuronal...
Arginine side‐chains play a distinct role because of their high pKa and perpetual positive charge. An NMR method is presented, based on carbon‐detected 13Cζ–15Nε correlation spectra, which allows probing the arginine side‐chains and their dynamics at neutral‐to‐high pH. The methodology ...
图8 同一骨架的抗体,HCDR3带正电比带负电更容易结合各种pI的无关蛋白。抗体B2=+2,AF1=-5,5DA是AF1的变体,HCDR3的net charge为0。 图9 137个获批或临床中的抗体在pH=7.4下的CDR净电荷,与抗体特异性的关系。 Arginine诱导抗体非特异性在人工合成抗体库中被进一步证明。曾经在Genentech任职的Sidhu擅长设计minim...
Biochemistry A 'facultatively' essential amino acid that contains a guanido group with a pKa > 12, which carries a positive charge at physiological pH; it becomes an essential amino acid when the body is under stress or injured Sources Turkey, chicken and other meats. See Unproven methods for...
5. Although the methylated arginine residue retains its positive charge, the ability to form hydrogen bonds is reduced, probably affecting the protein-protein interaction. In addition, arginine methylation is very stable compared to that of other PTMs, and hence, its kinetics are less dynamic2,5...
(The letter y is derived from lysine, the first substrate described for this system, and the + signifies the positive charge of the AA substrates.) Additional features of system y+ are pH insensitivity and strong stimulation by hyperpolarization and by substrate at the opposite membrane side (...
When PIP2 replaces PS in the membrane at equivalent charge densities, the EPR lineshapes and power saturation data also indicate that this region contacts the bilayer. As seen in Supplementary Fig. 1, site 350 makes a closer approach and site 285 is further away from the interface in PIP2...