此外,限制异亮氨酸摄入可以降低葡萄糖耐受性和胰岛素敏感性,提示限制蛋白质摄入产生的积极代谢效应需要对异亮氨酸进行限制才能实现。Arg-1和鸟氨酸脱羧酶(ornithinedecarboxylase)ODC1能促进巨噬细胞持续的胞葬作用,通过Rac1的激活来促进胞浆的HuR蛋白(humanantigen R)的表达,进而结合并稳定了编码Dbl的mRNA。
1.检测CAC组织和健康对照组织中Arg-1的表达水平:选取20例CAC患者组织和20例健康对照组织,使用免疫组织化学方法检测Arg-1在组织中的表达水平,并采用实时荧光定量PCR(qRT-PCR)方法检测Arg-1在组织中的mRNA水平。 2.探讨Arg-1在CAC中的作用及其影响机制:使用CRISPR-Cas9技术构建Arg-1敲除细胞株,在体外培养中模拟炎症...
Arg-1iNOS探讨胶原诱导的关节炎(collagen-induced arthritis,CIA)小鼠发病过程中骨髓和脾脏扩增的髓样抑制细胞(myeloidderived suppressor cells,MDSC)产物Arg-1,iNOS的mRNA表达水平.应用牛II型胶原免疫DBA/1J小鼠诱导CIA模型,在第一次免疫后的第45天,采用流式细胞术分选小鼠骨髓和脾脏CD11b^+Gr-1^+的MDSC(纯度〉...
分类号:密级: UDC:编号: 学位论文 不同基因型弓形虫成囊株感染大鼠腹腔巨噬 细胞诱导Arg-1合成机制的研究 TheMechanismsofArginase-1SynthesisinRatPeritoneal MacrophagesInfectedwithCyst-formingStrainsofT. gondiiwithDifferentGenetypes 张倩 指导教师姓名**龙教授安徽医科大学病原生物学教研室 申请学位级别硕士专业名称临...
Total RNAs were isolated with an RNeasy Mini Kit (Qiagen) and first-strand cDNA was synthesized by reverse transcription of mRNA using oligo(dT)21 primer and SuperScriptTM III Reverse Transcriptase (Invitrogen). Real time PCR was performed using the following primers: Myc, 5′-GAGCAGCAGAGAAA...
Absin还提供一些独有的特色产品:霍乱毒素B亚单位(CTB)、鸡胚提取物(CEE)、牛脑垂体提取物、呼吸爆发试剂盒、多色免疫组化试剂盒、 mRNA原料等在业界深受客户喜欢。 Absin秉承踏实,务实,求精和不断创新的精神为中国科学家提供优质产品,努力打造属于中国自己的生物试剂品牌,为广大科学家提供更全面更丰富更优质的产品。
1、温故知新|肝脏过氧化物体增殖物激活受体γ基因启动子 甲基化及其 mRNA 表达下调降低 胎儿生长受限大鼠胰岛素敏感性 2、温故知新|宫内生长受限大鼠肝组织胰岛素样生长因子-2与过氧化物酶体增殖物激活受体-γ辅激活因子-1α表达的相关...
The molecular consequence of this translocation is the generation of a chi- meric Bcr/c-Abl mRNA encoding activated Abl protein tyrosine kinase. The related protein tyrosine kinase Arg, also designated Abl2, contains an SH2 and an SH3 domain. Arg has been shown to interact with and to ...
KMO mRNA levels and activity in prefrontal cortex (PFC) are reduced in schizophrenia. We hypothesized that KMO expression in PFC would be reduced in bipolar disorder with psychotic features and that a functional genetic variant of KMO would associate with this disease, CSF KYNA level and KMO ...
The mRNA expressions of NLRP3, caspase1 and IL-1β was quantified. Using primers were listed in the Supplemental Table 1. All data was normalized to the mRNA expression level of β-actin. 2.7. Cytoplasmic DNA isolation and mtDNA copy number analysis Mitochondria were extracted from macrophage ...