Cosmetics In Vitro Skin Sensitisation Assay- ARE-Nrf2 Luciferase Test Method首页 标准 T/SHRH 014-2018 标准全文 T/SHRH 014-2018 预览[下载] 解读 仪器设备 50 仪器谱 50 发布历史T/SHRH 014-2018点击打开全屏PDF预览 点击查看大图 标准号 T/SHRH 014-2018 发布 2018年 总页数 11页 发布单位 中国团体...
___ 化妆品 体外皮肤致敏试验-ARE-Nrf2 荧 光素酶试验方法 Cosmetics In Vitro Skin Sensitisation Assay- ARE-Nrf2 Luciferase Test Method 2018-12-01 发布 2018-12-30 实施 上海日用化学品行业协会 发布 目次 前言……… 2 1.范围……… 3 2.规范性引用文件………...
ARE-Nrf2LuciferaseTestMethod2018-12-01发布2018-12-30实施上海日用化学品行业协会发布1目次前言………21.范围………32.规范性引用文件………33.术语和定义………34.原理………45.测试系统………46.
Therefore, we developed a simple and highly accurate KE-2 test method named 伪-Sens that uses the dual luciferase assay system and attempted a further application of luciferase-based determination of cell viability to calculate the normalized Antioxidant response element (ARE)-mediated transcriptional ...
In direct opposition to the effects obtained by overexpressing NRF2, KEAP1 overexpression led to a significant down-regulation of xCT promoter activity when MCF-7 cells were co-transfected with an xCT promoter-luciferase construct (Fig. 3A, p<0.05). KEAP1 overexpression, which was confirmed at ...
Additionally, the TargetScan predicted that SIRT6 was a target of miR-370 and confirmed by luciferase activity assay. Moreover, miR-370 inhibited SIRT6 expression and regulated Nrf2/ARE signal pathway, whereas overexpression of SIRT6 partly reversed the effect of miR-370 on OGD/R-induced SH-...
Next, we aimed to determine the effect of salicylate on metastases formation of CRC cells in mice. For this we utilized SW620 CRC cells stably expressing luciferase (SW620-Luc2). We have previously shown that mature miR-34b and miR-34c are expressed at very low levels in SW620-Luc2cells...
Therefore, we used the luciferase reporter assay to measure the promoter activity of ARE. The luciferase activity derived from the ARE promoter consistently increased after MitoQ treatment in a dose-dependent manner (data not shown). These data suggest the possible involvement of Nrf2 signaling in ...
After incubation at 37°C for 20 min, the absorption of each test sample was evaluated at 450 nm. The SOD activity was calculated in accordance with the instructions in the kit. 2.12. Signal Pathway Detection by Dual-Luciferase Reporter Assay The following seven cytokines were examined in...
This genomic pathway (also called “natural antioxidant cell defense”) is well-known to increase cell capacity to adapt to oxidant stress or aggression (Ma, 2013) and the Nrf2-dependent luciferase reporter gene assay has been successfully used to revealed the activation of the expression of Nrf2...