The disk was placed onto a blood agar plate (Merck), carrying a 5 ml nutrient soft agar overlay with 200 μL exponentially growing S. pneumoniae cells (OD600 = 0.2). Plates were incubated overnight. The following day, the kill zone diameter was manually measured. PenG-only, PenG...
Mice were monitored over 80 hours for survival or killed 24 hours after infection to harvest blood for cytokine measurements and organs. Bacterial burden was determined as described previously17 by plating homogenates of lungs and livers onto blood-agar plates and counting bacterial colonies after 18...
Factor VII (FVII) is a single-chain serine protease zymogen that circulates in blood and can bind the receptor tissue factor (TF) with high affinity and specificity. When bleeding occurs, TF in the blood vessel wall is exposed to FVII. Once bound to TF, FVII is activated into FVIIa, fo...
Varying amounts of cell suspension corresponding to known amounts of cellular protein were applied to duplicate wells of ELISA plates, and immunoassayed for the presence of Lewis X or Lewis Y antigen as described in Methods. Negative control samples of E. coli HB101, or buffer-only blanks, ...
Cell adhesion, mediated by apically and latero-basally found junctional buildings, is a must for steady attachment of these cells to each other as well as the basal lamina. Whereas former studies have focused on the formation and remodelling of this cellular connections in the apical area, righ...
After 1 hour incubation, plates were washed with PBS and biofilms were removed from plates by sonication using a washer IS-4 (InterSonic) for 15 minutes. After that, samples were diluted 10–1000-fold and 10 μL aliquots of each dilution were plated on MacConkey agar a...
In 1986, Beppu and colleagues found that the antibiotic trichostatin A inhibited the growth of SV40-transformed cells in mice [7], one of the first examples of selective growth inhibition by a HDAC inhibitor. Two compounds, vorinostat and romidepsin, have been approved by the FDA to treat refr...
After incubation overnight the microbes were plated on YPD agar plates to determine colony forming units (CFU) or an XTT assay was performed as shown in Figure S4. For assays in Figure 4 A & B C. albicans and NETs were incubated at 30°C. Immunodepletion of calprotectin from NETs Twenty...
Sheep’s blood agar (Hardy), VRE chromID (bioMerieux), Spectra MRSA (Remel), HardyCHROM ESBL (Hardy), MacConkey agar with cefotaxime (Hardy), Cetrimide agar (Hardy), and Sabouraud dextrose + chloramphenicol (Hardy). Plates were incubated at 35 °C in an air incubator and incubated...
At least 10 test crosses between one male and one female of each strain (SB372 and SB347) were attempted. Crosses were attempted in both directions (SB372 female and SB347 male, and vice-versa). Although males and females engaged in mating behavior, no embryos were observed in plates. Th...