Moreover, we found that overexpression or knockdown of ARAP1-AS2 could regulate fibrosis and HK-2 cell proliferation through EGFR/TGF-尾/Smad3 signalling. RNA pulldown assays revealed that ARAP1-AS2 directly interacts with ARAP1. Coimmunoprecipitation, dual-immunofluorescence and ubiquitination assays...
使用ARAP1-AS2过表达质粒探讨ARAP1-AS2是否通过调控ARAP1参与高糖诱导的HK-2细胞损伤,以期发现DN发生的新分子机制,为糖尿病肾小管损伤的临床治疗提供新策略。方法:1、将HK-2细胞分为正常糖组(NG)和高糖组(HG),培养48小时后,RT-PCR检测lncRNA-ARAP1-AS2,ARAP1 mRNA水平变化,Western blot及免疫荧光检测ARAP1的...
ARAP1-AS2表达逐渐上调,其正义链靶基因ARAP1(Arf GAP with Rho GAP domain,ankyrin repeat and PH domain1)m RNA的表达逐渐上调.但是目前,天然反义lncRNA-ARAP1-AS2对其正义链靶基因ARAP1的调控机制,以及天然反义lncRNA-ARAP1-AS2调控ARAP1介导糖尿病肾脏损伤的机制尚不清楚.天然反义lncRNA-ARAP1-AS2及其正义...
The aim of this work was to dissect a novel regulatory mechanism of Yin and Yang 1 (YY1) on lncRNA-ARAP1-AS2/ARAP1 to regulate EGFR/PKM2/HIF-1α pathway and glycolysis in DKD. Materials and methods: We used adeno-associated virus (AAV)-ARAP1 shRNA to knocked down ARAP1 in diabetic...
目的:分析健康对照组,糖尿病组,糖尿病肾病组患者血清mRNA-ARAP1(ArfGAP with RhoGAP domain,ankyrin repeat and PH domain1,锚蛋白重复和PH结构域1)及LncRNA-ARAP1-AS1(ArfGAP with RhoGAP domain,ankyrinrepeat and PH domain1 antisense RNA1,锚蛋白重复和PH结构域1反义RNA1),LncRNA-ARAP1-AS2(ArfGAP with...