L-arabinose operonOver forty years of research on the l-arabinose operon of Escherichia coli have provided insights into the mechanism of positive regulation of gene activity. This research also discovered DNA looping and the mechanism by which the regulatory protein changes its DNA-binding properties...
Genetic analysis of the arabinose operon was begun by Ellis Englesberg. His studies were among the first to suggest that a regulatory protein could act positively to turn on expression of a gene rather than act negatively to turn off expression as had been shown for the lac and lambda phage...
A DNA fragment containing the l-arabinose operon regulatory region of Escherichia coli was purified from DNA heteroduplexes formed between opposite strands of two non-defective ara transducing phage. The phage and arabinose gene orientation is such that the heteroduplex contains two single-stranded ...
The pBAD system is based on thearabinoseoperon. This is induced by adding arabinose, which binds to the AraC regulatory protein. Activated AraC exits the O2site and binds to the I site (Fig. 10.9). This activates transcription of the cloned gene. The pBAD system is modulated by the amoun...
They form a common regulatory system: a regulon. The activation of the ara operon not only requires the presence of the substrate arabinose, but also that the catabolite activating protein cyclic adenosine monophosphate complex be attached to the promoter. This operon is subject to catabolite ...
The human sex-linked genes RPS4X and RPS4Y encode distinct isoforms of ribosomal protein S4. Insufficient expression of S4 may play a role in the developme... AR Zinn,RK Alagappan,LG Brown,... - 《Molecular & Cellular Biology》 被引量: 128发表: 1994年 A Ribosomal RNA Operon and its ...
This system exhibits an increase in heterologous protein production upon induction with L-arabinose and comprises a mutant araB promoter and an AraC transcription binding region. This system retains the tight regulatory control characteristic of the wild type arabinose operon.Tanja Maria Gruber...
Figure 2.Regulatory control features of the ara operon. Sites for RNA polymerase (RNAP), AraC, and cyclic AMP repressor protein (CRP) binding within the ara control region are shown schematically. AraC protein binds to each of the three binding sites,aral,araO1, andaraO2, as a protein dime...
The coding regions may individually be expressed from promoters, or two or more coding regions may be joined in an operon with expression from the same promoter. The resulting chimeric genes may be introduced into Zymomonas cells and maintained on a plasmid, or integrated into the genome using,...
High-affinity L-arabinose transport operon: nucleotide sequence and analysis of gene products. J. Mol. Biol. 197:37-46.Scripture, J.B. , Voelker, C. , Miller, S. , O'Donnell, R.T. , Polgar, L. , Rade, J. , Horazdovsky, B.F. , and Hogg, R.W. 1987 . High-affinity L-...