(Invitrogen, Carlsbad, California, USA) according to the manufacturer's recommendation, when treated with conditioned media of Z3 cells or BMMS-03 cells.The cells were harvested 48 h after transfection, and the activities of target gene luciferase and renilla luciferase were determined with the ...
San Francisco: University of California San Francisco; 1998. 1 0 Lin SS, Cai WM, Rui JZ. The new theory and new method for therapeutic drug monitoring. Beijing: Publishing House of Chi- nese Medicine; 2002. 1 1 Jonsson EN, Karlsson MO. Xpose. An S-PLUS based population pharmacokinetic/...
Fetal calf serum (FCS), anti-Nup88 and anti-Nup98 antibodies were purchased from Santa Cruz (California, USA). Fluorescein isothiocyanate (FITC)-labeled secondary antibodies were purchased from Zhongshan Company (Beijing, China). Chemiluminescence (ECL) reagent kits were purchased from Pierce ...
After 3 washes in PBS, the secondary antibody (CY3-conjugated goat antirabbit, FITC-conjugated goat antirabbit, Santa Cruz Biotechnology, Santa Cruz, California, USA) was used at a 1:200 dilution in 3% BSA, and the incubation lasted for 1 h at room temperature. The nuclear localization of...
Based on the known ternary complex, the docked structure was used for CoMFA study after the above-mentioned compounds were docked into the complex model using molecular simulation program Dock4.0 (Department of Pharmaceutical Chemistry, Univer- sity of California, San Francisco, USA). The enzyme/...
The PCR fragments were cloned into the pGEM-T vector for DNA sequencing and then transferred to pTrcHIS (Invitrogen, San Diego, California, USA) series for expression. Recombinant proteins were purified using Co2+– Sepharose. Cell maintenance and proliferation assays Breast can- cer cells MDA-MB...
A ZORBAX Eclipse XDB- C18 column (particle size, 5 µm; 4.6×150 mm; Agilent, California, USA) was used as an analytic column. The mo- bile phase was a mixture of 0.625% acetic acid and acetoni- trile (85:15, v/v) for chlorogenic acid, 0.625% acetic acid and methanol (75:...