The present invention relates to the discovery of a toxin-antitoxin system in opportunistic human pathogenand to the applications of this discovery including the stabilization of plasmids useful in the field of recombinant DNA technology for production of genes and their products. The Phd-like (...
The Functional Annotation of Prokaryotic Taxa (FAPROTAX,http://mem.rcees.ac.cn:8080/) pipeline was used to extrapolate bacterial community functions relating to nutrient cycling (i.e., C, N, and S in the study) (Djemiel et al.2022; Ling et al.2022; Louca et al.2016). For P cyclin...
Conventional poly-and monoclonal antibodies are still indispensable reagents in basic research and diagnostics. Nevertheless, both of them have some shortcomings-most of all the batch-to-batch variability of the polyclonal antibodies and the elevated costs and long time necessary for the production of ...
the pH-responsive molecules eliminated the toxin significantly faster than the conventional molecules. Recycling and sweeping antibodies theoretically can remove antigens out of circulation even if they do not neutralize them in in-vitro assays. Thus, they can be used as efficient antitoxins when a ...
A major aspect of microbial metabolic engineering is the development of chassis hosts that have favorable global metabolic phenotypes, and can be further engineered to produce a variety of compounds. In this work, we focus on the problem of decoupling growth and production in the model bacteriumEsc...
Other bacterial ense systems that have been studied or discovered by NGS include the toxin/anti-toxin, antigen, novel restriction-modification, and DNA phosphorathioation systems as well as those involved with infection-induced dormancy or programmed cell death [192]. Genomic sequencing also has ...
3. The neutralizing monoclonal antibody or the antigen-binding fragment thereof of claim 1 or 2, wherein the neutralizing monoclonal antibody or the antigen-binding fragment thereof binding immune-specifically with tetanus toxin, and the neutralizing monoclonal antibody or the antigen-binding fragment th...
“Toxin-Antitoxin Genes of the Gram-Positive Pathogen Streptococcus pneumoniae: So Few and Yet So Many” Microbiology and Molecular Biology Reviews, 2012, 76(4):773-791. Chang, et al. “Genome editing with RNA-guided Cas9 nuclease in Zebrafish embryos” Cell Research, 2013, 23:465-472. ...
is., H. alvei) containing endogenous plasmids that encode specific toxin/antitoxin gene pairs. Such toxin/antitoxin gene pairs play a role in maintenance of the genetic information and response to stress. (See, Wertz et al. “Chimeric nature of two plasmids of Hafnia alvei encoding the ...
To overcome this, the bacteria have evolved with the production of a molecule, referred to as antitoxin, to negate the deleterious effect of the toxin against itself. The toxin and antitoxins are encoded by a two-component toxin-antitoxin (TA) system. The antitoxin, a protein or RNA, ...