These results show that beta-sitosterol potently induces apoptosis in U937 cells and that beta-sitosterol-induced apoptosis is related to the selective activation of caspase-3 and induction of Bax/Bcl-2 ratio. 展开 关键词: Humans U937 Cells Sitosterols Proto-Oncogene Proteins c-bcl-2 Apoptosis ...
One of the earliest structural changes in the dying cell is widespread chromatin condensation, of the type characteristic of apoptosis, the mode of death frequently observed where cell deletion seems to be `programmed'. It is shown here that this morphological change is closely associated with ...
RNA-seq analysis revealed that expression of the Clusterin (CLU) gene, which is related to apoptosis, was downregulated by ascorbate. The suppression of CLU was verified at transcript level in different melanoma cell lines, and at protein level in A2058 cells. The anti-apoptotic cytoplasmic CLU...
It is well-known that BCL-2 family proteins are major regulators of apoptosis11. Growing evidence suggest that the BCL-2 family members perform other functions in addition to their canonical role in apoptosis, including mitophagy12. BCL-B (also called BCL2L10, Boo and Diva) is the most rec...
PCD must be tightly controlled since disregulated cell death is involved in the development of a large number of different pathologies. To counter the multitude of processes that are capable of triggering death, cells have devised a large number of cellular processes that serve to prevent ...
Musk is widely used in clinical practice for its anti-cancer properties. Here, we treated various types of cancer using musk to determine which cancers are sensitive to musk treatment. We also compared effects of native musk and synthetic musk ketone in cancer cells. Furthermore, we investigated...
Besides, platelet size is thought to be positively associated with platelet reactivity [15], inferring that platelet enlargement may be a compensation for the weakened hemostasis during thrombocytopenia. Unfortunately, the mechanisms that regulate platelet size and reactivity, especially in the context of...
Although genistein, a natural isoflavonoid phytoestrogen, has been shown to have pro-apoptotic activity against human cancer cell lines, little is known about the mechanism of genistein in terms of TRAIL-induced apoptosis. In the present study, it was investigated whether or not combined treatment ...
To verify if ROS generation is related to HVJ-E-induced cellular apoptosis, some cells were treated with NAC, an ROS scavenger. To perform this assay, PC3 cells were incubated with NAC (100 μmol/L) for 30 min prior to HVJ-E treatment. Collected cells were analyzed by flow cytometry (...
Thep53signaling pathway is also involved in cell apoptosis. Therefore, we analyzed apoptosis-related gene expression afterSESN1overexpression or knockdown. At the same time, the protein levels of Cleaved-Caspase 3, Cleaved-Caspase 8, and Cleaved-Caspase 9 were also elevated. Overexpression ofSESN1...