Flow Cytometry: Principles and Applications. Totowa, NJ: Humana Press. pp. 147-163.Allen, P., & Davies, D. (2007) Apoptosis detection by flow cytometry. In M. G. Macey (Ed.), Flow cytometry: Principles and applications (pp. 147). Totowa, NJ: Humana Press Inc....
• By using a fluorochrome labelled inhibitor peptide that binds to the active site of the caspase; • By using a non-fluorescent substrate for the enzyme which yields a fluorescent product if the enzyme is active. Other changes Other methods can also be used in the detection of apoptosis...
RNA Detection By Flow Cytometry Microbiology Assays for Flow Cytometry 5 Steps to Intracellular Flow Cytometry Immunophenotyping Kits for Flow Cytometry There are many different apoptosis assays for flow cytometry ...
for flow cytometry because cell death cascades are complex and dynamic, underscoring the importance of a multi-parametric approach to the assessment of apoptosis. Since no single parameter defines programmed cell death, a combination of techniques is reco...
翻译结果3复制译文编辑译文朗读译文返回顶部 Detection of myocardial cell apoptosis by flow cytometry 翻译结果4复制译文编辑译文朗读译文返回顶部 Myocardial cells apoptosis detection by flow-cells 翻译结果5复制译文编辑译文朗读译文返回顶部 The cardiac muscle cell perishes the examination to pass the overflow typ...
The apoptosis of human hyperplastic prostate tissues were studied by TUNEL detection, flow cytometry and agarose gel electrophoresis. The DNA sequence of ... X Gu,J Ning,Q Ma,... - IEEE 被引量: 2发表: 0年 Flow Cytometry,Imaging Flow Cytometry and Fluorescent Microscopycombining the Power of...
Detection of apoptosis by flow cytometry THP-1 cells (1 × 106 cells/well) were seeded in 6-well plates, transfected with siRNA-DUSP1, incubated for 24 h, and infected with BCG (MOI = 10) and incubated for another 6 h. Apoptosis was detected by FACS using an Annexin V/propi...
damage may occur during cell detachment or harvesting. Methods for utilizing Annexin V for flow cytometry on adherent cell types, however, have been previously reported (Casiola-Rosen et al. and van Engelend et al.). INDUCTION OF APOPTOSIS BY CAMPTOTHECIN ...
When cells reached 50–60% confluence, they were incubated with DMSO/SC79 (4 μg/ml) for 24 h for apoptosis detection28,29. Determination of apoptosis by flow cytometry The pMEFs were incubated with DMSO/SC79 (4 μg/ml) for 24 h. Apoptotic cells were identified using the ...
Investigators should note that the Annexin V-PE/7-AAD Apoptosis Detection Kit has not been routinely tested on adherent cell types. During cell detachment and harvesting, membrane damage may occur, and then bring about a false positive result. If an adherent cell type was used, it is recommend...