especially immune checkpoint blockade (ICB)18,19,20. Thus, mutation of either passenger or cancer-driving genes generates novel neoepitopes presented by tumor cells. These neoepitopes may be sufficiently different from the unmutated, self-epitopes to prime, ...
The single-stranded DNA cytosine-to-uracil deaminase APOBEC3B is an antiviral protein implicated in cancer. However, its substrates in cells are not fully delineated. Here APOBEC3B proteomics reveal interactions with a surprising number of R-loop factors. Biochemical experiments show APOBEC3B binding...
The cytidine deaminase, Apolipoprotein B mRNA editing enzyme catalytic subunit 3B (APOBEC3B, herein termed A3B), is a critical mutation driver that induces genomic instability in cancer by catalyzing cytosine-to-thymine (C-to-T) conversion and promoting replication stress (RS). However, the detai...
APOBEC3 (A3) cytidine deaminases induce mutations in single-stranded DNA substrates, in some cases, fatally hypermutating virus DNA and, in others, generating selectable mutations in chromosomal DNA of cancer cells. Some mechanisms by which virus-encoded factors modulate levels of A3 proteins are ...
Commons (GDC) data portal [37]. All CCLE WES data had been reported to be sequenced at the Broad Institute using the same version of the Agilent Exome Bait kit, and the same sequencing protocols and data processing pipeline were applied to all samples across all cancer categories [37,38]...
For example, a small ∼4-kb deletion in the A3B gene resulting in partial loss of A3B expression is found more frequently in breast cancer patients than in controls (77). Furthermore, in some patients, homozygous A3B deletions have been detected solely within the malignant tissue, ...
Masonic Cancer Center, Biostatistics and Bioinformatics Group, University of Minnesota, Minneapolis, Minnesota, 55455, USA Kevin AT Silverstein DIRO, Université de Montréal, Montréal, Quebec, H3C 3J7, Canada Mathieu Lajoie, Denis Bertrand & Nadia El-Mabrouk Department of Veterinary Microbiology and ...
CRISPR gene editing efficiency was verified using GeneArt Genomic Cleavage Detection kit (A24372; Thermo Fisher Scientific). A3B knockout cell lines were performed by transfection of the pSpCas9(BB)−2A-Puro (PX459) plasmid containing A3B gRNAs with FuGENE 6 Transfection Reagent (E2691; ...
In this study, the impact of the apolipoprotein B mRNA-editing catalytic subunit-like (APOBEC) enzyme APOBEC3B (A3B) on epidermal growth factor receptor (EGFR)-driven lung cancer was assessed. A3B expression in EGFR mutant (EGFRmut) non-small-cell lung c
The role of A3B in genome editing is intriguing, given that deletion of most of the A3B gene results in a higher odds ratio of developing breast, ovarian or liver cancer23–26. Indeed, complete genome sequencing of DA3B À / À breast cancer genomes revealed a higher than average ...