Fasting blood samples were collected after a 12 h fast prior to the beginning of the kinetic study in tubes containing disodium EDTA and benzamidine (0.03%) (33). Blood lipids were measured using enzymatic methods and ultracentrifugation as previously described (34). Glucose levels were measured ...
Blood samples were collected from the retro-orbital plexus and centrifuged at 2,500 g for 20 min at 4°C for plasma isolation. Five days after infusion, a subset of mice was killed and their livers harvested, frozen in liquid nitrogen, and stored at −80°C. LDL (1.019 < d < ...