The structure of apo and holo forms of xylose reductase, a dimeric aldo-keto reductase from Candida tenuis - Kavanagh, Klimacek, et al. - 2002 () Citation Context ... first improvement of ethanol fermentation from xylose by the protein engineering of XR. In the protein engineering of XR, ...
W6, for the same population, shows a very slight unfavorable interaction energy that varies with lipid type as well as between apo and holo forms. The external water population has little interaction with W6 and a stronger interaction with the apo than holo forms for W82 (not shown). The ...
Molecular Dynamics of a Thermostable Multicopper Oxidase from Thermus thermophilus HB27: Structural Differences between the Apo and Holo Forms Martiniano Bello*, Brenda Valderrama, Hugo Serrano-Posada, Enrique Rudin˜ o-Pin˜ era* Departamento de Medicina Molecular y Bioprocesos,...
Molecular Dynamics of a Thermostable Multicopper Oxidase from Thermus thermophilus HB27: Structural Differences between the Apo and Holo Forms Molecular dynamic (MD) simulations have been performed onTth-MCO, a hyperthermophilic multicopper oxidase fromthermus thermophilusHB27, in the apo as well ... ...
coli with good yields, thus allowing site-directed mutagenesis, and because it is the only flavodoxin for which the structures of both the apo and holo forms are known 因为再组合蛋白质可以用E.被表达。 杆菌与好出产量,因而允许站点被指挥的突变形成,并且,因为它是apo和holo形式结构知道的唯一的...
The ability to separate apo and holo forms of DBP should permit the study of their specific interactions with other binding proteins and help determine the physiological relevance of these interactions. 展开 关键词: protein separation liquid chromatography ...
Cofactor binding significantly stabilizes both proteins as revealed by the extension of secondary structures in the holo-forms, and the overall rigidity shown by the backbone dynamics data. However, the 50 s loops of both proteins in the holo-form still show conformat...
Molecular dynamics simulations of the apo-, holo-, and acyl-forms of Escherichia coli acyl carrier protein. J Biol Chem. 2008; 283 :33620–33629.Chan, D. I.; Stockner, T.; Tieleman, D. P.; Vogel, H. J. "Molecualr Dynamics Simulations of the Apo-, Holo-, and Acl-forms of ...
Cofactor binding significantly stabilizes both proteins as revealed by the extension of secondary structures in the holo-forms, and the overall rigidity shown by the backbone dynamics data. However, the 50 s loops of both proteins in the holo-form still show conformational exchanges on the 碌s-...
actionenergies,mobility,proteincontacttimes,andwatermolecule coordination.Anumberofdifferencesareobservedbetweentheapoand holo-formsandrelatedtodifferencesintheproteinstructure.Solvent insideapo-FABP,forexample,showscharacteristicsofawaterdroplet, whilesolventinholo-FABPbene®tsfrominteractionswiththeligand ...