subsequent experiments. We established the minimal boundary on labeling depth of APEX in acute slices using propargyl tyramide (PT), a clickable APEX substrate (Supplementary Fig.3c-d). Acute slices were incubated in ACSF supplemented with 250 µM PT for 1 h following the same protocol as...
By taking advantage of the unstable nature of the phenoxyl radical generated during oxidation of the reconstituted enzyme, we can obtain the limited interaction regions between proteins under a specific condition in live cells. Methods Chemicals and reagents PCDNA3-flag-APEX2 was purchased from Add...
Nature, 434 (2005), pp. 913-917 CrossrefView in ScopusGoogle Scholar Carreira and Kowalczykowski, 2011 A. Carreira, S.C. Kowalczykowski Two classes of BRC repeats in BRCA2 promote RAD51 nucleoprotein filament function by distinct mechanisms Proc. Natl. Acad. Sci. USA, 108 (2011), pp. ...
a specific inhibitor of rho-associated kinases. t ishizaki, m uehata, i tamechika, j keel molecular pharmacology 2000 2. a rock inhibitor permits survival of dissociated human embryonic stem cells k watanabe, m ueno, d kamiya, a nishiyama. nature biotechnology. 2007 product citation 1. jessic...
© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature About this protocol Cite this protocol Pla-Martín, D., Babatz, F., Schauss, A.C. (2023). Localization of Mitochondrial Nucleoids by Transmission Electron Microscopy Using the Tr...