Apaf-1是一种分子量为130kD的蛋白质,其存在于细胞质中。人Apaf-1基因位于染色体12q23。Apaf-1 由三部分特殊的结构域组成:①N-末端为85 个氨基酸残基,含有一个半胱氨酸蛋白酶募集域,称为CARD结构域,可结合N端具有同源性的caspase-9。②是和CED-4有很高同源性,含320个氨基酸残基的区域,CED-4同源域内包含一...
Apaf-1 由三部分特殊的结构域组成:①N-末端为85 个氨基酸残基,含有一个半胱氨酸蛋白酶募集域,称为CARD结构域,可结合N端具有同源性的caspase-9。②是和CED-4有很高同源性,含320个氨基酸残基的区域,CED-4同源域内包含一个保守的P环,与dATP/ATP结合,可促进自身的多聚化。③C-末端有12个WD-40重复片段,其阻...
APAF1 encodes a cytoplasmic protein that initiates apoptosis. Apoptotic protease-activating factor 1 contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. ...
G. (1999) Solution structure and mutagenesis of the caspase recruitment domain (CARD) from Apaf-1. Cell Death Differ. 6, 1125-1132Day CL, Dupont C, Lackmann M, Vaux DL, Hinds MG (1999) Solution structure and mutagen- esis of the caspase recruitment domain (CARD) from Apaf-1. Cell ...
In the presence of dATP and cytochrome c, the CARD domains of caspase-9 and Apaf-1 interact to produce an apoptosome complex in which procaspase-9 is processed to its active form, which in turn activates caspase-3 (10, 14, 24). The apoptosome has been defined as an Apaf-1-...
4. Shiozaki, E.N. et al. Oligomerization and activation of caspase-9, induced by Apaf-1 CARD. PNAS 99(7): 4197 Product References 1. Kamitsuji Y, Kuroda J, Kimura S, et al, The Bcr-Abl kinase inhibitor INNO-406 induces autophagy and different modes of cell death execution in Bcr-...
FIG. 1. Dominant negative caspase-9 (caspase-9-DN) blocked apoptosis in- duced by FADD, TRADD, and CED-4. A, a schematic presentation of Apaf-1. Apaf-1 contains an NH2-terminal CED-3- homologous region (amino acids 1- 89) that includes a CARD domain as indicated, a CED-4-homolog...
【题目】关于细胞凋亡的内源途径中,下列说法正确的是()(多)A.线粒体处于中心地位B.细胞色素C被释放到胞质与Apaf-1结合C.Apaf-1的caspase募集结构域(CARD)募集胞质中的caspase-3酶原,并自切割D.定位(大多数成员)在线粒体外膜上的Bcl-2蛋白家族的成员有的能促进细胞凋亡,有的能抑制细胞凋亡E.线粒体在 ...
Human CARD4 Protein Is a Novel CED-4/Apaf-1 Cell Death Family Member That Activates NF-κB The nematode CED-4 protein and its human homolog Apaf-1 play a central role in apoptosis by functioning as direct activators of death-inducing caspases. A ... J Bertin,WJ Nir,CM Fischer,... -...
¦[{º#,Apaf 1±"Mdê ÕZÝú7W`(Ñkl3 C©4²klú,O Apaf 1+g WD40RÒ[Çg C[\,Ü&ù 6 Apaf 1. ú Apaf 1g ¹ ù » Y / CARD © Ç O Caspase 9[\OUù¡,)*ê4;xklù¡F a[15].ÉZ¨×6g Apaf 1Où6g Caspase 9dù 6w...