Another potential way to decrease immunogenicity of CARs is to simplify the structure of the CAR’s antigen-binding domain by using heavy-chain-only binding domains25. Immunoglobulin-like molecules with antigen-binding domains made up of only heavy chains without light chains were first described in...
The antigen-binding specificity of an antibody, however, is mediated by a small proportion of the whole protein. These are the variable regions. They too consist of three to four different modules which are combined individually in each cell during the differentiation of B-lymphocytes. ...
the identification of the protein-protein interaction sites. In this study we report the use of synthetic peptides to the sequences in the amino-terminal region to probe for the pol δ region that is involved in its binding to PCNA. Additional experimental approaches using deletion mutants of ...
The combining site of the antibody is mostly flat with a protuberance made up of two tyrosines that penetrate the cleft. All six complementarity-determining regions of the Fab contribute at least one residue to the binding; one residue from the framework is also in contact with the lysozyme. ...
Antigen binding by B cell receptors (BCR) on cognate B cells elicits a response that eventually leads to production of antibodies. However, it is unclear what the distribution of BCRs is on the naïve B cell and how antigen binding triggers the first step in BCR signaling. Using DNA-PAIN...
(EBNA2). While type 1 EBNA2 is known to interact with human transcription factors (hTFs) such as RBPJ, EBF1, and SPI1 (PU.1), type 2 EBNA2 shares only ~ 50% amino acid identity with type 1 and thus may have distinct binding partners, human genome binding locations, and ...
The histochemical binding of four lectin-peroxidase conjugates to normal human gastric mucosa and gastric carcinoma is described. The lectins were peanut a... Selvaraj 被引量: 63发表: 1987年 Grading dysplasia in colorectal adenomas by means of the quantitative binding pattern determination of Arachis...
BORIS Binding to the Promoters of Cancer Testis Antigens, MAGEA2, MAGEA3, and MAGEA4, Is Associated with Their Transcriptional Activation in Lung Cancer Sheetal Bhan,Sandeep S Negi.BORIS binding to the promoters of cancer testis antigens,MAGEA2, MAGEA3 and MAGEA4, is associated with their tra...
(MHC) at its surface. These antigens are processed by antigen-presenting cells and presented as trimmed peptides held within the MHC-binding groove. The purpose of these peptide–MHC complexes is to communicate information to one of the major effectors of the immune system, T cells, which ...
(http://www.biokdd.fudan.edu.cn/Service/TEPITOPEpan/) to determine peptides with putative strong binding to HLA-I and HLA-II molecules. However, because peptide-HLA binding affinity and proteolytic cleavage also play key roles in determining the biological feasibility of miHA, the vast majority...