随着抗体药物偶联物(ADC,Antibody-drug Conjugate)技术的日益成熟,Genentech公司在2015年利用THIOMAB技术来特异性的偶联递送siRNA,即抗体-siRNA偶联物(ARC,Antibody-siRNA Conjugate),这是第一次尝试将siRNA直接偶联在抗体上,并且在小鼠的肿瘤模...
Antibody–siRNA conjugate Antibody–drug conjugate siRNA delivery Targeted delivery 1. Introduction Antibody–drug conjugates, which can target therapy agents to desired lesion areas, have achieved considerable market values [1]. Based on the fact that biological therapy agents such as small interfering ...
By creating that target C5 expression of that target C5aR antibody and siRNA of conjugate, the agitative signaling of two different aspects of therapeutic that target C5 of double mode.Nirmal Kumar BandaV. Michael Holers
1. (a) Synthetic Scheme for Conjugating an siRNA with a Linker; (b) Antibody–siRNA Conjugate Synthesis via Interchain Disulfide (Cysteine) Conjugation; (c) Antibody–siRNA Conjugate Synthesis via Asn297 Conjugation; (d) Antibody–siRNA Conjugate Synthesis via Lysine Conjugation We then partially re...
(缩写:gpp – glycopolypeptide; LYTAC – lysosome-targeting chimera; bsAb – bispecificAntibody; msAb – multispecific antibody; protac – proteolysis-targeting chimera;ARC – antibody-siRNA conjugate; ASO – antisense oligonucleotide; ADC –antibody-drug c...
further demonstrating the rationale of using antibodies as vehicles to specifically deliver siRNAs to nonliver tissues. In the late 2010s, Avidity Biosciences described an IgG-based antibody-siRNA conjugate strategy that achieved significant mRNA silencing in muscles and tumors, according to its published...
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® PTEN siRNA I (+), using PTEN Antibody #9552 and p42 MAPK (Erk2) Antibody #9108. PTEN Antibody confirms silencing of PTEN expression...
We synthesized a series of single- and double-stranded AOCs, as well as a human serum albumin-oligonucleotide conjugate, and studied their interactions with both targeted and non-targeted living cells using a time-resolved analysis of ligand binding assay. Our results indicate that conjugation of ...
scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Anti-SMYD3 Recombinant Rabbit Superclonal™ Antibody (Product # 711549, 1-2 µg/mL) and Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate ...View ...
Using intravenous administration, an antibody-siRNA conjugate system was able to specifically deliver siRNA into target cells by using the conjugated antibody. Based on the targeting capability of the antibodies, the ARC system was found to improve the efficacy of siRNA by reducing the required drug...