Although our in-house antibody screen was negative on automated solid-phase red cell adherence (SPRCA) testing, manual tube testing revealed the presence of anti-H antibodies, and forward typing with Ulex europaeus lectin confirmed the absence of H-antigen. Further, Lewis B phenotyping and saliva...
Antinuclear antibodies (ANAs) are valuable laboratory markers to screen for and support the diagnosis of various rheumatic diseases (known as ANA-associated rheumatic diseases). The importance of ANA testing has been reinforced by the inclusion of ANA positivity as an entry criterion in the 2019 sys...
To determine if the increased cross-reactivity to other α-neurotoxins translated into cross-neutralization, a single concentration antibody screen was set up using the Qube 384 system. Here, the three antibodies (368_01_C05, 2551_01_A12, and 2554_01_D11) were tested against α-cobratoxin f...
Positive antibody values have been observed in the negative control flock (Flock D), which had not been vaccinated and was free from chlamydial abortion. An explanation for the observations of an increasing antibody value amongst this flock is that the animals may have asymptomatic intestinal infecti...
MSAs and MAAs have a certain predictive value for the prognosis of DM [10]. In China, the largest hospitals began to fully screen for MSAs and MAAs in DM patients in 2016, and this study retrospectively analysed the records of 153 inpatients with DM. We analysed the positive rate of MSAs...
EBV IgM antibody was negative in 97 percent of recipients (n = 57) and 100 percent of donors (n = 67). Conclusion: The seroprevalence of EBV infection among candidate for kidney transplantation in khuzestan is not very high compared to other provinces in Iran, although we should perform ...
This counter-screen strategy facilitated the exclusion of GP mutants that were misfolded locally or that exhibited an expression defect (Davidson and Doranz, 2014). Generation of virus neutralization escape mutants To generate escape mutants, 100 PFU of EBOV-eGFP were combined with 2-fold dilution...
H-p21Ras, K-p21Ras and N-p21Ras were used to screen the hybridoma clones. Finally, the hybridoma KGH-R1, producing a broad-spectrum monoclonal antibody against the three p21Ras proteins, was isolated. Interestingly, the monoclonal antibodies reacted with both wildtype and mutated p21Ras in ...
A similar profile was noticed in vitro for soluble polyclonal rabbit anti-CRIA Ab. All together, our data suggest that a negative signaling in B cells may be initiated by surface Ig of their idiotypic partners subsequent to a strong cross-linking of their surface Ig receptors. 展开 ...
have isolatedhumanscFvantibodiesagainstsurfaceantigensofalungcarcinomacellline.Thesewereidentifiedfromapolyclonal mixtureofphagedisplay-enrichedlibraryclonescomparingPCRpatternsofthecarcinomacelllinewiththetwonegativecell types,HUVECandperipheralbloodcells(PBLs).ThepositiveclonesweresequencedandverifiedbyFACS. ©2007...