screen - a protective covering consisting of netting; can be mounted in a frame; "they put screens in the windows for protection against insects"; "a metal screen protected the observers" 3. screening - the act of concealing the existence of something by obstructing the view of it; "the ...
ELISA evaluation of the mAbs obtained by Ecobody technology. ELISA results for Zipbody mAbs produced in CFPS using Ecobody technology. The primary and secondary antibodies were six-fold diluted CFPS samples and 2000-times diluted anti-rabbit Ig(G + M)-HRP conjugate. NC means negative contr...
An automated solid phase antibody screen (SPAS) in microplates has been developed. Red blood cell (RBC) adherence was used as the end point instead of agglutination. Consequently, positive and negative reactions were readily distinguished by a microplate spectrophotometer. The SPAS performed as well ...
To determine if the increased cross-reactivity to other α-neurotoxins translated into cross-neutralization, a single concentration antibody screen was set up using the Qube 384 system. Here, the three antibodies (368_01_C05, 2551_01_A12, and 2554_01_D11) were tested against α-cobratoxin f...
FIGURE 1. Sandwich immunoassay for a true antigen-positive (a) and the mechanism of false-positive (b) and false-negative (c) interference. A circulating anti-animal antibody often arises as a result of the normal response of the immune system to the administered “foreign” protein antigen ...
(MCA-PSV). In the anemic fetus, the MCA-PSV increases as the hemoglobin decreases, a result of increased cardiac output, decreased blood viscosity andvasodilatationin the brain. An MCA-PSV value that is higher than 1.5 multiples of the median (MoM) is a screen for severe fetal anemia, ...
If true, our single-cell discovery platform could not only screen for functionality but also the “developability” (e.g., efficient expression) of candidate molecules in the same assay, thereby further accelerating downstream drug development. We will be particularly interested in evaluating if such...
This technology can screen for alterations in protein concentration and activation status relatively quickly. However, for this array type of assays and other types discussed below, such as single-cell western blotting and ChIP-on-Chip, intra-assay spatial variability must be addressed [23, 24]. ...
The other antibody that performed highly for immunocytostaining in AR-V7 positive 22RV1AR+/AR−V7+++ cells, clone SN8, caused however clearly noticeable nuclear staining in AR-V7 negative LNCapAR+/AR−V7− cells, interestingly of nucleolar appearance. This means use of this antibody for ...
The red circles and block indicate the positive controls (biotin-labeled BSA), and the green circles indicate the negative controls. d Diagram of mAb arrangement on the chip. e The peach mAbArray chip was used to screen for differentially expressed proteins in CN13 fruit between fruit-ripening...