As a consequence, all routine aggregation analysis methods, such as size-exclusion chromatography, cannot monitor the size distribution at those original conditions, but only after dilution and usually under completely different solvent conditions. In contrast, sedimentation velocity (SV) allows to ...
Small-angle X-ray scattering (SAXS) data have revealed an acid-induced reduction in the subpeaks in Kratky plot, indicating conformational changes that can lead to aggregation. The subpeaks are well resolved at pH > 3 but less pronounced at pH ≤ 2. One of the weakened su...
Thus they concluded that the recombinant construct provided superior ADCs compared to hinge disulfide conjugates in terms of ADC uniformity and aggregation levels [40]. 2.4 The Drug The choice of effective cytotoxic drugs for ADCs is governed by the relatively small number of antigen molecules on ...
Linker in ADC bridges the antibody with the cytotoxic drug. It is one of the key factors related to the stability of ADC and payload release profiles, and is therefore important for the ultimate therapeutic index of ADCs. An ideal linker should not induce ADC aggregation, and it is expected ...
A scFv antibody (scFv A4) was determined to inhibit aggregation and favor recovery of the native conformation of HCK during its refolding. This antibody also increased the stability of HCK during its heat-induced unfolding process. Our findings demonstrate that scFv A4 has dual-chaperone-like ...
Nevertheless, Petermann showed that crude papain could digest the antibody into fragments of roughly one-quarter of its original size (Petermann, 1946), while Porter (1950) found that the products of antiovalbumin antibody fragmentation inhibited ovalbumin aggregation mediated by intact antiovalbumin ...
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Compound74is a class of NAMPTi-ADCs with low aggregation and high selectivity, obtained from the same payload and linker but different antibodies (LYPD3, c-erbB2, CD276), reported by Böhnkeet al. in 2022. Compound74exhibited remarkable potency, with an IC50value of 7 pm in THP-...
We use essential cookies to make sure the site can function. We also use optional cookies for advertising, personalisation of content, usage analysis, and social media. By accepting optional cookies, you consent to the processing of your personal data - including transfers to third parties. Some...
The Pneumococcal Serine-Rich Repeat Protein Is an Intra-Species Bacterial Adhesin That Promotes Bacterial Aggregation In Vivo and in Biofilms The Pneumococcal serine-rich repeat protein (PsrP) is a pathogenicity island encoded adhesin that has been positively correlated with the ability of Strept......