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Methods Blood samples from CONV and VAC were collected one year after infection or MVA-BN vaccination (single-dose course in previously primed with historical smallpox vaccination (hSV), two-dose course in non-primed). MPXV-specific IgG and neutralizing antibodies (NAb) titers were measured by ...
Where possible, blood was collected from active patients prior to commencement of immunosuppression therapy. Four (24%) and twenty three (28%) of the active and remission patients with AAV were on no immunosuppression at the time of sampling. Antibodies against MPO and PR3 were determined by ...
Rabbit anti-vaccinia virus antibody (Abcam) and anti-rabbit Alexa Fluor Plus 555 (Thermo Fisher Scientific) were used as the primary and secondary antibodies, respectively. Nuclei were visualized using 4,6-diamidino-2-phenylindole (DAPI), and fluorescence was visualized using a fluorescence ...
A total of 200 suspected patient there was120 infected with the parasite diagnosed by the direct examination method, a blood sample was taken from each one, as well as (60) healthy controls were involved. The study included the measurement of Anti-neutrophil cytoplasmic antibodies c-ANCA (Anti...