1. AntimiR-21局部注射抑制miR-21的表达 作者首先确定短暂栓塞下miR-21的表达情况,发现短暂阻断左前降支(60分钟)后,左心室心肌的远区和边缘区的miR-21表达逐渐上调(图1A)。接着进行缺血/再灌注(I/R),5天内不进行治疗以形成疤痕,分别在I/R后第5天和第19天进行将LNA修饰的antimiR-21注入冠状动脉(图1B),...
Background miR-21 is a central regulator of cardiac fibrosis, and its inhibition in small-animal models has been shown to be an effective antifibrotic strategy in various organs, including the heart. Effective delivery of therapeutic antisense micro鈥搑ibonucleic acid (antimiR) molecules to the ...
Development of macrophage-targeted carbohydrate-coupled antimiR-21 (RCS-21) We next aimed to identify candidate cell surface molecules on pulmonary macrophages that may allow cell type-specific targeting and delivery of microRNA inhibitors to these cells (see Fig. 2a for candidate stratification). Tr...
aWe found that miR-16 and miR-21 were upregulated upon nicotine stimulation, transfection with anti-miR-16 or anti-miR-21 significantly abrogated cell proliferation. 我们发现miR16和miR21是upregulated在尼古丁刺激、transfection与反miR16或反miR21显著被废止的细胞增殖。[translate]...
Effect of 2鈥 -sugar and phosphate backbone modifications on hybridization and anti-miR-21 activity of ASOsScott, DavisBridget, LolloSusan, FreierChristine, Esau
1B,C). To downregulate miR-21 in tumors cells, we packaged a lentiviral (LV) miRzip anti-sense miR-21 to stably express RNAi hairpins and investigated the efficacy of miRzip-21 mediated downregulation in different tumor cell types (Fig. 1D). RT-PCR analysis on LV-miRzip-21 transduced...
Anti-miR21 oligonucleotide enhances chemosen- sitivity of T98G cell line to doxorubicin by inducing apoptosis. Am J Cancer Res 5: 231-242, 2015.Giunti L, da Ros M, Vinci S, Gelmini S, Iorio AL, Buccoliero AM, et al. Anti-miR21 oligonucleotide enhances chemosensitivity of T98G cell line...
antimiR-21超声引导靶向治疗胰腺癌发展恶化极快,加上非常容易产生化学治疗耐受,因此治疗颇为棘手.近年来发现这些均与microRNA(miR)-21高表达有关.antimiR-21是miR-21的抑制剂,含有与miR-21互补的核苷酸序列,与miR-21杂交使miR-21无功能从而达到治疗效果.文章阐述了miR-21在胰腺癌恶化中的作用机制及应对方案;在...
本发明提供了一种肿瘤曲妥珠单抗靶向antimiR21纳米粒子,为抗肿瘤曲妥珠单抗靶向载药高分子负载antimiR21的纳米粒子,所述抗肿瘤曲妥珠单抗靶向载药高分子的结构式如式(I)所示.本发明还提供了上述肿瘤曲妥珠单抗靶向antimiR21纳米粒子的制备方法.该肿瘤曲妥珠单抗靶向antimiR21纳米粒子靶向性好,毒副作用小,其制备方法工艺...
Nedaeinia R,Sharifi M,Avan A,et al.Locked nucleic acid anti-miR-21 inhibits cell growth and invasive behaviors of a colorectal adenocarcinoma cell line:LNAanti-miR as a novel approach[J].Cancer Gene Ther,2016,23(8):246-253.Nedaeinia, R.; Sharifi, M.; Avan, A.; Kazemi, M.; ...