Anti-HMGB1 neutralizing antibody ameliorates neutrophic airway inflammation by suppressing dendritic cell-mediated Th17 polarization. Mediators of Inflammation, 2014.Zhang F, Huang G, Hu B, Fang LP, Cao EH, Xin
Recently, we found that the translocation and release of HMGB1 from neuronal nuclei to the extracellular space occurs both in ischemic and traumatic brain injuries, and that a neutralizing monoclonal antibody (mAb) against HMGB1 significantly ameliorates the resultant brain injuries by protecting against...
Tokyo, Japan) according to the manufacturer’s instructions. Anti-HMGB1 antibody (Lifespan Biosciences, Nottingham, United Kingdom ) was used to block HMGB1 in the tumor cell SNs for selected experiments.
We also applied a neutralizing antibody to HMGB1 and validated the function of neutralizing cytokine, as shown by blocking the induction of TNF-α in Mϕs (Figure 4D). Given the validated recombinant mouse (rm) HMGB1 HMGB1 and anti-HMGB1, we cultured CD8 T cells from both wild-type (WT...
During ischemia reperfusion (IR) injury, high mobility group box 1 (HMGB1), a chromatin binding protein, is released from necrotic cells and triggers inflammatory responses. We assessed the therapeutic effect of a neutralizing anti-HMGB1 monoclonal antibody (mAb) on lung IR injury. A murine ...
The ability of extracellular HMGB1 to induce its own neutralizing antibody suggests a feedback loop limiting the level of this DAMP in a healthy body. Currently, our studies are ongoing to identify and characterize the human counterpart B cells that produce the anti-HMGB1 IgM, by first ...