An ELISA for the detection of anti-HLA class I IgG antibodies (PRASTAT TM ) was developed and compared to microlymphocytotoxicity (Figure 1). ELISA plates were coated with a panel of soluble HLA (sHLA) antigens isolated from the culture supernatants of 46 different EBV transformed phenotyped...
Specificity:class I HLA-A, B and Cw antigens. Isotype:Human IgG1, kappa Formulation:0.2 µm filtered solution in PBS with 5% saccharose Applications:positive controls for all anti-HLA antibody detection assays, including: multiplex bead array assays using Luminex® technology (LABScreen®, LI...
链接:https://www.antibodysystem.com/product/14750.html 名称:Anti-Human HLA-A/B/C Antibody (HB95) (MHM00101)抗体 别名:HLAA, Human leukocyte antigen A, HLA class I histocompatibility antigen, A alpha chain, HLA-A, HLA-B, HLA class I histocompatibility antigen, B alpha chain, Human leukoc...
Aim: to analyze the relationship between circulating anti-HLA antibodies (class-I and class-II), renal pathology and C4d deposition in cases of late acute and chronic antibody-mediated renal allograft rejection according to the method of antibodies detection (ELISA and LUMINEX). Materials and ...
Anti-Human HLA-A, B, C Monomorphic (Clone W6/32) recognizes a monomorphic determinate (α chain) of major histocompatibility HLA Class I antigens. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype....
If the patient had DSAs and no alternative donors, a DS was employed with rituximab (day -15), 2 single volume plasmaphereses (PP; days -9 and -8), intravenous immunoglobulins (day -7) and infusion of HLA selected platelets, if persistent DSAs were directed against class I HLA. DS ...
De novo Anti-HLA Antibody Responses after Renal Transplantation: Detection and Clinical Impact Numerous retrospective and prospective studies have been conducted to determine the prevalence and significance on long-term graft survival of de novo post... M Seveso,E Bosio,E Ancona,... - 《Contribution...
1. Clinical implications of positive posttransplant DSA detection according to DSA status: preformed or de novo DSAs STAR 2017 recommended that anti–HLA antibody assessment be performed using a solid-phase assay and includes all major HLA class I and II loci (HLA-A, -B, -C, -DRB1, -DRB...
2.2. Detection of Anti-HLA Antibodies In order to quantify anti-HLA class I and class II antibodies, sera from 97 patients with a positive panel reactive antibody test (>5%) were further analyzed with two SAB tests, LIFECODES® Single Antigen Assay (Immucor GTI Diagnostics, Inc, Waukesha, ...
Table 1. Diverse HLA class-I isoform reactivity of the monoclonal antibodies generated against two alleles of recombinant HLA-E heavy chains. HLA-A Negative Negative Negative Negative A*1101+ Positive A*1101+ Positive A*1101+ Positive Negative Negative Negative Negative Negative A*1101+ Positive ...