TUBE(Tandem Ubiquitin Binding Entities, 串联泛素结合实体),基于已知具有对泛素亲和力的蛋白质结构域,经过深度整合,创造性地开发出了TUBEs结合实体。与单泛素结合相关结构域(UBA)相比,TUBE对多泛素部分的亲和力增加高达1000倍。 此外,TUBEs对多泛素化蛋白质具有保护作用,允许以相对低的丰度进行检测。 这些特性有助于有...
串联结合实体Tandem Ubiquitin Binding Entities(TUBEs)的开发和使用正在成为泛素研究不可或缺的策略,是多聚泛素分离和表征的革命。FLAG标记的Anti-K63 TUBE是从细胞和组织提取物或体外合成混合物中有效分离和富集K63-多聚泛素的理想试剂。其由多个泛素蛋白相互作用基序(UIM)组成,与其它链接类型相比,K63-TUBE对K63链...
Anti-K48 TUBE, His6 Cat. # UM205 来自 lifesensors.com 喜欢 0 阅读量: 81 作者: Altun,HB Kramer,LI Willems,JL Mcdermott,CA Leach,SJ Goldenberg,KG Kumar,R Konietzny,Fischer,E Kogan 展开 摘要: Based on protein domains known to possess an affinity for ubiquitin, Tandem Ubiquitin Binding...
USP5 simultaneously removes both K48-linked unanchored and K63-linked anchored polyubiquitin chains on IRF3. Our study not only provides evidence that unanchored ubiquitin regulates anti-RNA viral innate immunity but also proposes a novel mechanism for DUB-controlled I...
5A), supporting the potential AR-E2 ubiquitin-conjugating function of UBE2J1. Considering that the most common pattern of ubiquitination associated with protein degradation is lysine 48 (K48)-linked ubiquitination [34,35,36], we conducted a co-IP of AR in wildtype LNCaP/AR and UBE2J1-KO ...
translocation [130]. N-terminal 1-361 amino acids are sufficient for this IFN antagonism [130]. ORF3a protein upregulates SOCS1, an E3 ligase that negatively regulates IFN signaling, which dampens STAT1 phosphorylation and accelerates JAK2 degradation in a ubiquitin-proteasome-dependent manner [...
Anti-Ubiquitin (linkage-specific K48) antibody [EP8589] - BSA and Azide free重组Anti-Ubiquitin (linkage-specific K48)抗体[EP8589] - BSA and Azide free Anti-SRP54 antibody [EPR10851(B)] - BSA and Azide free重组Anti-SRP54抗体[EPR10851(B)] - BSA and Azide free ...
CircMAN1A2 competes with FBXW11 for binding to SFPQ, preventing FBXW11-mediated K48-linked SFPQ ubiquitination and protein degradation To further investigate which E3 ubiquitin ligases mediate SFPQ ubiquitination regulated by circMAN1A2, we performed a co-IP experiment in HEK293T cells using an SF...
The latter targets the K63-linked ubiquitylation of TRAF6 to limit the phosphorylation of IκBα to prevent its K48-linked ubiquitylation and its degradation by the ubiquitin-proteasome to block the nuclear translocation of NF-κB. Klebsiella also activates NOD1 upon inhibition of RAC1 to induce ...
However, TRABID specifically disassembles K29- and K33-linked ubiquitin chains36 as well as K29/K48 branched ubiquitin chains37 and therefore would be unfavorable to reverse the action of APCCdh1, which assembles K11-containing branched ubiquitin chains63,64. Thus, USP35 and TRABID may ...