(D) Relative expression levels of Rab7 and Rab9 in the SKBR-3 cells treated with trastuzumab, M32 or H32 IgG were analyzed by Western blot, where GAPDH was detected as internal control. The Western blots were derived under the same experimental conditions from the same cell lysates; the ...
The expression levels of ERK, phosphorylated ERK, and p21 were detected by western blot. β-actin expression was used as a loading control. the increased toxicity limits their application. Based on this, the rationale underlying the development of target- ing drugs has shifted from focusing ...
Western blot analysis from whole cell lysates (20 μg of proteins/lane) were immunoblotted using anti-ERK1/2, -p-ERK1/2, -STAT3, -p-JNK1/2/3, -c-Myc, -H2AX, -p-H2AX, -H3, -p-H3, -mTOR, and -p-mTOR antibodies. GAPDH served as a loading control. Results are ...
The other part of the colon was snap-frozen for quantitative real-time polymerase chain reaction (Real-Time PCR) and Western blot. The macroscopic damage score was calculated based on the extent of inflammation along the colon (one point for each centimeter of moderate inflammation or two points...
4.12. Protein Extraction and Western Blot Analysis RAW264.7 cells were seeded at a density of 5 × 106 cells/dish in 10 cm dish and then with different concentrations of SS-1 (125, 250 and 500 µg/mL) and 100 ng/mL of LPS for 24 h to measure the protein expression levels. The ...
2.8. Protein Extraction and Western Blot Analysis After differentiation, the cells were washed twice with ice-cold Dulbecco's phosphate buffered saline (DPBS) and lysed in lysis buffer containing a protease inhibitor cocktail (Roche Applied Nutrients 2018, 10, 1221 4 of 12 Science). The cell ...
Western blot analysis of tumor samples after PDT revealed a striking upregulation of iNOS and also a 1400W-inhibitable increase in the level of NO-derived nitrite [45]. This was the first reported evidence for iNOS upregulation by PDT in vivo using a human tumor model and for iNOS/NO-...
We also evaluated insulin sensitizing potential of test compounds using 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxyglucose (2-NBDG) glucose uptake assays in insulin-resistant HepG2 cells and PTP1B protein expression using the western blot technique. 2. Results 2.1. Inhibitory...
4.8. Detection of Caspase-3 Activated by Western Blot SH-SY5Y cells were seeded at 1 × 106 cells/mL density into a Petri dish and incubated for 24 h. After treatments with HTyr, HTyr-OL, OA, and vinblastine at their respective EC50 concentrations, cells were lysed with NP40 buffer on...
To demonstrate the characteristic of EVs, the same number of EVs (1.78 × 108 particles) were analyzed by Western blot. EVs were incubated with RIPA buffer for 30 min and centrifuged for 10 min at 4 ◦C. The proteins of EVs were separated on 10% SDS-PAGE and transferred to ...