Therapeutic efficacy of a MMAE-based anti-DR5 drug conjugate Oba01 in preclinical models of pancreatic cancer Article Open access 29 April 2023 A therapeutic antibody targeting annexin-A1 inhibits cancer cell growth in vitro and in vivo Article Open access 10 January 2024 Back...
e, f, UV–visible (e) and MALDI–TOF (f) analyses reveal that the drug-to-antibody ratio of trastuzumab–SEC–MMAE is approximately 4. g, h, HPLC–QTOF mass spectrometry analysis of MMAE released from trastuzumab–SEC–MMAE after incubation with Phe-BF3 for 3 h (magenta), 15 h (blue...
The fluorescence intensity of FITC was determined using flow cytometer (Novocyte, ACEA Bioscience, San Diego, CA, USA). The following formula was used to calculate the internal- ization efficiency rate of each agent in cells: internalization efficiency rate (%) = [(fluorescence intensity of the...
Protein G-MMAE Protein G-MMAF Protein G-Duocarmycin Protein G-Amanitin Protein G-Amanitin Rabbit Anti-vc-PAB-MMAE pAb Mouse Anti-vc-PAB-MMAE mAb (B11F11) Rabbit Anti-MMAF pAb Mouse Anti-MMAF mAb (F3B11) Mouse Anti-MMAE / F mAb (B12A2) Mouse Anti-MMAE /F mAb (C12A1) Rabbit An...
respectively. Cells were washed three times with PBS and then labeled with Albumin Antibody-FITC conjugate (Thermo Scientific) at 4 °C for 30 min. After incubation, cells were washed three times and resuspended in 500 μl PBS. The fluorescence intensity of FITC was determined using flow...