Learn more about Antibody Enhanced Validation concentration ~2 mg/mL technique(s) immunocytochemistry: 20-40 μg/mL using transfected 293Tcells expressing luciferase immunocytochemistry: suitable western blot: 2-4 μg/mL using whole extract of transfected 293T cells expressing luciferase ...
Thermo-life PA532207 ANTIBODY FOR NS1 (DENGUE 2) EA ￥3,613.00 EA Thermo-life PA532208 ANTI-FIREFLY LUCIFERASE EA ￥3,613.00 EA Thermo-life PA532209 ANTI-FIREFLY LUCIFERASE EA ￥3,613.00 EA Thermo-life PA532210 ANTI-RENILLA LUCIFERASE EA ￥3,613.00 EA Thermo-life PA532211 ANTI-NON-STRUC...
The Fzd receptor antagonistic antibody OMP-18R5 and the Wnt ligand trap OMP-54F28 both inhibit Wnt ligand induction of β-catenin activation in 293T-TCF/LEF luciferase reporter assays (Figures 2A and S1A) (Fischer et al., 2017; Gurney et al., 2012; Jimeno et al., 2017; Moore et al...
sites (EBS; A/CGGAA) (Supplementary Fig.5b) present on the proximal TM promoter was shown by luciferase reporter assays (sequence of the TM promoter construct shown in Supplementary Fig.6). ERG overexpression resulted in transactivation of TM wild-type (TM WT) promoter in HUVEC (Fig.5c); ...
Luciferase cell expression was confirmed 72 h post infection using the IVIS200 system (Invitrogen). Bioluminescent cellular cytotoxicity with jurkat. Platelets were harvested from whole blood as described above and incubated either with PD-L1 neutralizing antibody (BPS Bioscience, Cat. No. 71213...
TC71 cells have been engineered to express a red-shifted Luciola italica luciferase transgene, and the obtained TC71 Luc cells were tested by IVIS, resulting in a photon emission of 1 774 ± 582 photons/second (p/s) per cell (data not shown). The bioluminescent signal after luciferin ...
Methods The expression of CEACAM1 on T-lymphocytes in the peripheral blood of patients with glioma was detected before and after radiotherapy; GL261 murine glioma cells (stably transfected with the luciferase gene) were implanted in the right caudate nucleus of C57BL/6 mice, and tumour ...
(Fig.3A). We used a luciferase reporter assay to determine the relevance of the NBRE sequence in theRasGRP1gene for the regulation of the Nurr1 mediated inflammation signaling. TheRasGRP1promoter, followed by an 850 base pair region surrounding the intron site with NBRE, was cloned upstream ...
After 5 to 16 h (as indicated in results), Bio-Glo™ Luciferase Assay Substrate (Promega) was added to the wells and luminescence was measured using the Infinite® 200 microplate reader (Tecan). Estimated EC50 values were calculated by on-linear regression on log-transformed data using ...
the anti-CD27 antibody or antigen-binding fragment thereof comprising a light chain variable region and a heavy chain variable region, said light chain variable region comprising a complementarity determining region light chain 1 (CDRL1) amino acid sequence ...