泛素蛋白(Ub)是存在于所有真核细胞中的高度保守的8kDa多肽,通过三种酶的顺序作用与靶蛋白中赖氨酸残基的ε-氨基缀合,E1Ub活化酶,E2偶联酶和E3连接酶。另外,Ub内的7个赖氨酸本身可以作为Ub受体,导致形成多聚泛素蛋白链。在这些K48和K63连接中有很好的特征。K48连接的多聚泛素化靶向用于蛋白酶体降解的蛋白质,...
泛素蛋白(Ub)是存在于所有真核细胞中的高度保守的8kDa多肽,通过三种酶的顺序作用与靶蛋白中赖氨酸残基的ε-氨基缀合,E1Ub活化酶,E2偶联酶和E3连接酶。另外,Ub内的7个赖氨酸本身可以作为Ub受体,导致形成多聚泛素蛋白链。在这些K48和K63连接中有很好的特征。K48连接的多聚泛素化靶向用于蛋白酶体降解的蛋白质,...
虽然单个Ub与靶蛋白的缀合被称为单泛素化,但是另外的Ub部分可以与该初始Ub缀合,形成多聚泛素(polyUb)链。这些polyUb链通过Ub的C末端与靶蛋白的赖氨酸之间的异肽键形成而连接。PolyUb链本身通过各个单体Ub单元之间的类似缀合机制形成,并且已经显示通过Ub中存在的所有赖氨酸(K6,K11,K27,K29,K33,K48和K63)连接...
E HEK293T cells were transfected with SFPQ, FBXW11 and HA-Ub (K48 or K48R) and subjected to ubiquitin assay. F Co-IP assays confirmed that circMAN1A2 inhibited the binding between FBXW11 and SFPQ. G HA-Ub, myc-FBXW11, along with flag-SFPQ (WT and its point mutants) were co-...
N. et al. Insights into ubiquitin chain architecture using Ub-clipping. Nature 572, 533–537 (2019). Article ADS CAS PubMed PubMed Central Google Scholar Yu, Y. et al. K29-linked ubiquitin signaling regulates proteotoxic stress response and cell cycle. Nat. Chem. Biol. 17, 896–905 (...
(Myc-tagged KEAP1) and PD-L1-SF, following with the treatment of 10 μM MG132 for 6 hours. Immunoblotting with Myc and Flag antibodies.CUbiquitination assay of PD-L1 in HEK293T cells co-transfected with Myc-KEAP1, PD-L1-SF, Ub-HA, or K48-HA and K48R-HA with the ...
P, phosphorylation; M, methylation; A, acetylation; green Ub, K63 polyubiquitination; cyan Ub, Met1 linear polyubiquitination; brown ub, K48 polyubiquitination. See Table S1 for details on bacterial effectors. (B) Modulation of host intracellular trafficking to promote intracellular niche ...
WT Ub and its mutants (K6, K11, K27, K29, K33, K48, and K63) were subcloned in pCMV with a HA tag. The lentiviruses for expression of shRNAs or exogenous genes were packaged in 293 T cells with standard packaging plasmids and method. Lentiviral infection of the cells was ...
The single-, double-, and triple-sites mutants of Myc-CPSF6 were co-transfected with FLAG-SYVN1 and HA-K48-Ub in HEK293T cells, followed by VSV infection and western blot analysis with the indicated antibodies. (C) HEK293T cells were co-transfected with FLAG-SYVN1, HA-K48, and WT...
泛素蛋白(Ub)是存在于所有真核细胞中的高度保守的8kDa多肽,通过三种酶的顺序作用与靶蛋白中赖氨酸残基的ε-氨基缀合,E1Ub活化酶,E2偶联酶和E3连接酶。另外,Ub内的7个赖氨酸本身可以作为Ub受体,导致形成多聚泛素蛋白链。在这些K48和K63连接中有很好的特征。K48连接的多聚泛素化靶向用于蛋白酶体降解的蛋白质,...