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中文名称:ANTI-HA TAG RABBIT POLYCLONAL ANTIBOD英文名称:ANTI-HA TAG RABBIT POLYCLONAL ANTIBOD 产品类别:抗体 一抗 WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, ...
单克隆抗体多元弧菌流式细胞术Objective] To establish the flow cytometry(FCM) method for detecting mul-ti-vibrio based on anti-OmpW monoclonal antibody.Methods] With the rate of cell stainning as an indicator,optimize the reactived conditions of anti-OmpW mAb concentration and reaction time for ...
Cells were stained with an anti-HA AF488 antibody (ThermoFisher, clone 16B12, A21287) for 1 h following fixation and permeabilization with 0.01% Triton for 10 min at room temperature. Cells were counterstained with DAPI and sealed with vecta shield (Vector labs). Images were acquired ...
产品名称:anti-human CD4 Antibody,FITC 产品别名:CD4抗体 宿主:Mouse 反应种属:Human 克隆性:Monoclonal Antibody 保存方法:protected from light at 2–8 °C. Do not freeze. 产品描述: 用途范围:Flow cytometry 产品信息订购: 产品货号产品名称规格价格大包装及货期 ...
The phenotype and function of immune cells in tumor microenvironment were characterized with multi-color flow cytometry. Tumor stemness was detected by ALDH assays. To characterize antigen-antibody binding capacity, we determined the dissociation constant of selected anti-FABP4 antibodies via surface ...
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography Application Notes Application Flow cytometry Routinely Tested Immunohistochemistry-paraffin Tested During Development Immunohistochemistry-zinc-fixed Tested During Development ...
antibody and a matched isotype control at 4 °C for 30 min in the dark. After washing three times with PBS, the cells were analyzed using flow cytometry (Beckman Coulter Cytoflex) and data were analyzed using CytExpert V2.3 and FlowJo X software. The value of PD-L1-FITC median ...
Calcium dye-loaded CD8+ T cells were coated with α-CD3 (10 μg/ml; OKT3) for 15 min on ice, washed with ice-cold PBS and activated with goat anti-mouse Fab’2 secondary antibody at 37°C (13 μg/ml) before flow cytometry analysis of calcium influx. Confocal Microscopy Eight-well ...
Nanocarrier-based antibody targeting is a promising modality in therapeutic and diagnostic oncology. Single-walled carbon nanotubes (SWNTs) exhibit two unique optical properties that can be exploited for these applications, strong Raman signal for cancer