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Here, using monoclonal anti-CD20 antibody (rituximab) as an example and the CD20+ Raji cell line as tumor target, we describe flow cytometry-based assays for determining an antibody's capacity for mediating antibody-dependent cellular cytotoxicity (ADCC), antibody-dependent cellular phagocytosis (...
et al. PD-1 blockade by CT-011, anti-PD-1 antibody, enhances ex vivo T-cell responses to autologous dendritic cell/myeloma fusion vaccine. J. Immunother. 34, 409–418 (2011). CAS PubMed PubMed Central Google Scholar Son, C. H. et al. CTLA-4 blockade enhances antitumor immunity of...
Flow cytometry and cell sorting After blocking nonspecific binding with Fc-blocking antibody 2.4G2 by incubation for 10 min on ice, single-cell suspensions were stained with surface-labeled antibodies (see Supplementary Table 1) for 20–30 min at 4 °C and then were washed with MACS bu...
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However, immune cells (e.g., CD8 + T cells and NK cells) showed impaired effector functions, with reduced or no killing of MM cells in an in vitro flow cytometry assay, compared with patients who responded to treatment [33]. Investigations in larger numbers of anti-CD38 antibody-...
产品名称:anti-human CD4 Antibody,FITC 产品别名:CD4抗体 宿主:Mouse 反应种属:Human 克隆性:Monoclonal Antibody 保存方法:protected from light at 2–8 °C. Do not freeze. 产品描述: 用途范围:Flow cytometry 产品信息订购: 产品货号产品名称规格价格大包装及货期 ...
英文名称:Anti-VEGF-C Polyclonal Antibody产品类别:抗体 来源用途交叉反应性理论分子量实际分子量 RabbitWB, IHCH, M, R46kDa47kDa WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Ass...
(f) Timeline of the treatment protocol used for the mouse model of ovalbumin (OVA)-induced allergic airway inflammation. (g) Gating strategy of the flow cytometry analysis of cells derived from the BALF of mice during ovalbumin-induced allergic airway inflammation (corresponding to data in Fig. ...
We determined this first by undertaking whole-protein lysate western blot analyses with a pan-lysine glutarylation (K-glutarylation) antibody36,38. We found that protein glutarylation is clearly detectable in CD8+T cells (Fig.3b). We also found that glutarylation patterns change strikingly after...