Chapter Seven - Affinity Pull-Down of Proteins Using Anti-FLAG M2 Agarose Beads 热度: Anti-Digoxigenin-AP, Fab fragments - Sigma-Aldrich 热度: Sigma_Flag-M2 beads 热度: MonoclonalANTI-FLAG®BioM2−Biotin,CloneM2producedinmouse,purifiedimmunoglobulin ...
crosslinked4%agarosebeads. EZviewRedANTI-FLAGM2AffinityGelfunctionsto bindFLAG ® fusionproteinsfromcelllysatesandother biologicalsamplesinthesamemannerasthe standardnon-coloredANTI-FLAGM2Monoclonal AntibodyAgaroseAffinityGel(CatalogNumber A2220).FLAGfusionproteins,boundtotheANTI- FLAGAffinityGel,arerecoveredby...
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Anti-FLAG M2 Magnetic Beads are 4% agarose beads bound with the exclusive Sigma Anti-FLAG M2 (mouse monoclonal) antibody.
For immunoprecipitation, cell lysates containing an equal amount of proteins were incubated with anti-Flag agarose beads (Sigma-Aldrich), GFP-Trap agarose beads (Chromotek), or antibody-conjugated Protein A Magnetic beads (Merck) at 4 °C for 2 h. The beads were washed with lysis buffer...
For IP-Mass spectrometry experiments, MC3T3-E1 cells stably overexpressing mouse Osteolectin-Flag were lysed and immunoprecipitated with anti-Flag M2 affinity gel (Sigma) overnight at 4°C. The beads were washed three times with RIPA buffer and eluted using 100 μg/ml 3X FLAG peptide in ...
The rest of the supernatant was incubated with 5 µL of pre-washed and pre-blocked Anti-FLAG® M2 Magnetic Beads (Sigma-Aldrich, M8823) (blocked with 3% BSA in PBS for 30 min at 4 °C with constant rotation) overnight at 4 °C with constant rotation. The next day the...
The desired protein was precipitated with appropriate antibodies conjugated directly to Sepharose beads (such as Flag M2 beads, Sigma) or via protein A/G-conjugated Sepharose beads. About 2 mg total protein worth of cell lysates were incubated with the antibody-conjugated beads for 1 h at ...
C-terminal DYKDDDDK-tagged green renilla luciferase protein was expressed in a HeLa IVT system (Cat. No. 88892) and immunoprecipitated using Pierce Anti-DYKDDDDK Magnetic Agarose or Sigma Anti-FLAG™ M2 Magnetic Beads using the KingFisher Flex Purification System. Tagged proteins were competitively ...
Mouse monoclonal ANTI-FLAG M2 affinity agarose gel (A2220) and Mouse monoclonal anti-HA-agarose (A2095) were obtained from Sigma-Aldrich. The antibodies used for FACS were as follow: anti-CD45 (30-F11, #103140, BioLegend); anti-CD3 BV785 (17A2, #100232, BioLegend); anti-CD3ε BV...