Anti-FLAG M2 Magnetic Beads are 4% agarose beads bound with the exclusive Sigma Anti-FLAG M2 (mouse monoclonal) antibody.
Anti-FLAG M2 Magnetic Beads are 4% agarose beads bound with the Anti-FLAG M2 (mouse monoclonal) antibody. The M2 antibody recognizes the FLAG sequence at the N-terminus, Met-N-terminus and C-terminus. This alows for detection and capture of fusion proteins containing a FLAG peptide sequence...
Chapter Seven - Affinity Pull-Down of Proteins Using Anti-FLAG M2 Agarose Beads 热度: sigma anti-FLAG F9291 热度: X3850 M2X3950 M2 内存配置规则 热度: 相关推荐 EZviewRedANTI-FLAG ® M2AffinityGel CatalogNumberF2426 StorageTemperature−20°C TECHNICALBULLETIN ProductDescription EZviewRed...
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Chapter Seven - Affinity Pull-Down of Proteins Using Anti-FLAG M2 Agarose Beads 热度: Anti-Digoxigenin-AP, Fab fragments - Sigma-Aldrich 热度: 6+sigma培训资料 热度: MonoclonalANTI-FLAG®BioM2−Biotin,CloneM2producedinmouse,purifiedimmunoglobulin ...
The rest of the supernatant was incubated with 5 µL of pre-washed and pre-blocked Anti-FLAG® M2 Magnetic Beads (Sigma-Aldrich, M8823) (blocked with 3% BSA in PBS for 30 min at 4 °C with constant rotation) overnight at 4 °C with constant rotation. The next day the...
C-terminal DYKDDDDK-tagged green renilla luciferase protein was expressed in a HeLa IVT system (Cat. No. 88892) and immunoprecipitated using Pierce Anti-DYKDDDDK Magnetic Agarose or Sigma Anti-FLAG™ M2 Magnetic Beads using the KingFisher Flex Purification System. Tagged proteins were competitively ...
HEK 293 cells were transfected with FLAG-tagged IκBα, IKKα, IKKβ, and TAK1 expression plasmid (1 μg) using the OmicsFect for 48 h. Total cell lysates were pulled down with anti-FLAG M2 agarose beads for 3 h and the pulled-down proteins were subjected to extensive washing ...
For IP-Mass spectrometry experiments, MC3T3-E1 cells stably overexpressing mouse Osteolectin-Flag were lysed and immunoprecipitated with anti-Flag M2 affinity gel (Sigma) overnight at 4°C. The beads were washed three times with RIPA buffer and eluted using 100 μg/ml 3X FLAG peptide in ...
In brief 100–200 µg/200–300 µl of total protein either from whole cell lysate or from cytoplasmic and nuclear fractions was incubated overnight with 20 µl of packed volume of M2 beads in a cold room under slow rotation. After washing repeatedly we used 3×FLAG peptide (Sigma, ...