Sigma_Flag-M2 beads 热度: Upconversion and Anti-Stokes Processes with f and d Ions in Solids 热度: Chapter Seven - Affinity Pull-Down of Proteins Using Anti-FLAG M2 Agarose Beads 热度: MonoclonalANTI-FLAG ® BioM2−Biotin,CloneM2
For immunoprecipitation, cell lysates containing an equal amount of proteins were incubated with anti-Flag agarose beads (Sigma-Aldrich), GFP-Trap agarose beads (Chromotek), or antibody-conjugated Protein A Magnetic beads (Merck) at 4 °C for 2 h. The beads were washed with lysis buffer...
The affi nity-purifi ed ANTI-FLAG M2 antibody displays virtually exclusive selectivity for the target protein band in Western blot immunostaining in these expression systems. We also demonstrate the utility of affi nity-purifi ed ANTI-FLAG M2 antibody for indirect immunostaining of cells that are ...
Anti-FLAG M2 Magnetic Beads are 4% agarose beads bound with the exclusive Sigma Anti-FLAG M2 (mouse monoclonal) antibody.
The rest of the supernatant was incubated with 5 µL of pre-washed and pre-blocked Anti-FLAG® M2 Magnetic Beads (Sigma-Aldrich, M8823) (blocked with 3% BSA in PBS for 30 min at 4 °C with constant rotation) overnight at 4 °C with constant rotation. The next day the...
Total cell lysates were pulled down with anti-FLAG M2 agarose beads for 3 h and the pulled-down proteins were subjected to extensive washing to purify the FLAG-fusion proteins by excluding any bound proteins in the pulled-down complexes. To determine whether DUSP5 directly bind to IκBα,...
Anti-FLAG M2 (F1804) and anti-β-actin (A1978) antibodies were purchased from Sigma-Aldrich (St. Louis, MO). Anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH, HRP-60004) antibody was from Proteintech (Rosemont, IL). Anti-Lamin A (sc-20680) and anti-HA tag (sc-805) antibodies were...
After centrifugation at 14,000 rpm for 10 min at 4 °C, the supernatants were incubated with Flag-agarose beads (A2220, Sigma-Aldrich) at 4 °C overnight. After that, the beads were washed five times with NETN buffer and the immunoprecipitates were collected and boiled in 50 μL of ...
The desired protein was precipitated with appropriate antibodies conjugated directly to Sepharose beads (such as Flag M2 beads, Sigma) or via protein A/G-conjugated Sepharose beads. About 2 mg total protein worth of cell lysates were incubated with the antibody-conjugated beads for 1 h at ...
Mouse anti-FLAG M2 Sigma-Aldrich Cat# F3165; RRID:AB_259529 Rabbit anti-c-Myc Sigma-Aldrich Cat# C3956; RRID:AB_439680 Mouse anti-HSP 70 Santa Cruz Biotechnology Cat# sc-32239; RRID:AB_627759 Mouse anti-Lamin B1 Santa Cruz Biotechnology Cat# sc-56143; RRID:AB_2136302 HRP goat anti-...