Use of Computer-Aided Immunofluorescence Microscopy (CAIFM) for Interpretation of Antinuclear Antibody (ANA) Pattern and Titerantinuclear antibodyautoimmune diseasedigital pathologycomputer-aided immunofluorescence microscopyThe assessment of antinuclear antibodies (ANA) by indirect immunofluorescence (IFA) allows ...
a) Homogeneous pattern on IFA b) Most commonly seen in drug-induced lupus c) In drug-induced lupus there is not normally n association with other autoantibodies 15 Anti-Nuclear Antibody (ANA) 4) Anti-RNP antibodies (Speckled or Nucleolar Pattern) ...
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ANA analysis, the IFA method using Hep-2, and liver cells (Euroimmun, Germany) was performed and the results were reported by an experienced specialist in microbiology. Clinical data were obtained from hospital records. For each connective tissue disease and the IFA pattern, ANA titers were ...
During the last decade, the International Consensus on ANA Patterns (ICAP) initiative promoted harmonization and understanding of HEp-2 IFA staining pattern nomenclature, as well as promoting their use in patient care by providing interpretation for HEp-2 IFA test results. In conjunction with a ...
Some of these are outlined in this article, including the planning of three audio-visual educational modules to be posted at the www.anapattern.org website, the classification of two novel HEp-2 IFA patterns, the implementation of a project dedicated to continuously updating the information on ...
A total of 172 consecutive ANA-IFA positive serum specimens, including the four most common pattern types, were evaluated in parallel by two readers to determine the dilution endpoint. The two methods produced equal dilution endpoints in 78 specimens (45%), within 1-dilution endpoint in 121 ...
The antinuclear antibody (ANA) test has high sensitivity in diagnosing and classifying systemic lupus erythematosus (SLE).#To describe the immunological pattern of SLE patients through investigating specific antinuclear autoantibodies by enzyme dot immunoassay and studying their frequency in both positive ...
Moreover, IL-17A levels were elevated in the sera with AC-4 IFA pattern when compared to AC-29 (15.6 [4.4-28.9] vs 2.5[0.35-8.45], p=0.033). When opposing patients 'sera, positive for only one ANA, with "simple" AC-IFA pattern, to sera with mixed AC pattern (positive for more ...
The pattern recognition also showed only minor aberrant findings resulting in a slightly better detection of cytoplasmic and nuclear membrane patterns by system A while system B detected slightly better the centromeric pattern.Disclosure of InterestNone declared...