Nevertheless, more recently the combination of HEp-2 IFA with SPA seems to be of added value [3]. One advantage of HEp-2 IFA is the recognition of immunofluorescence patterns, not only restricted to the nucleus, but also including the cytoplasm and the mitotic apparatus. This pattern provides...
During the last decade, the International Consensus on ANA Patterns (ICAP) initiative promoted harmonization and understanding of HEp-2 IFA staining pattern nomenclature, as well as promoting their use in patient care by providing interpretation for HEp-2 IFA test results. In conjunction with a ...
在线看3. How to Read an ANA IFA Pattern – 3 Steps 3分钟 28秒。2018 11月 8的高清视频,VK免费视频库免注册!
ANA analysis, the IFA method using Hep-2, and liver cells (Euroimmun, Germany) was performed and the results were reported by an experienced specialist in microbiology. Clinical data were obtained from hospital records. For each connective tissue disease and the IFA pattern, ANA titers were ...
(ANA) I.ANAScreeningTests 1)IndirectImmunofluorescence a)Hep-2cellsubstrate 1.Humancancercellline b)Incubatepatientserumoncellsubstrate c)Wash d)Incubatesubstratewithfluorescent-conjugated anti-humanantibody e)Observestainingpatternunderfluorescent microscope 4 Anti-NuclearAntibody(ANA) 1.IncidenceofANAin...
Use of Computer-Aided Immunofluorescence Microscopy (CAIFM) for Interpretation of Antinuclear Antibody (ANA) Pattern and TiterFluorescent Antinuclear AntibodiesImmunofluorescence MicroscopyAutoimmune Diseasecomputer-assistedinterpretationThe assessment of antinuclear antibodies (ANA) by indirect immunofluorescence (IFA)...
ANA analysis, the IFA method using Hep-2, and liver cells (Euroimmun, Germany) was performed and the results were reported by an experienced specialist in microbiology. Clinical data were obtained from hospital records. For each connective tissue disease and the IFA pattern, ANA titers were ...
Moreover, IL-17A levels were elevated in the sera with AC-4 IFA pattern when compared to AC-29 (15.6 [4.4-28.9] vs 2.5[0.35-8.45], p=0.033). When opposing patients 'sera, positive for only one ANA, with "simple" AC-IFA pattern, to sera with mixed AC pattern (positive for more ...
The antinuclear antibody (ANA) test has high sensitivity in diagnosing and classifying systemic lupus erythematosus (SLE).#To describe the immunological pattern of SLE patients through investigating specific antinuclear autoantibodies by enzyme dot immunoassay and studying their frequency in both positive ...
SAT0560Automated Ifa Methods Compare Well with Established Manual IFA Screening and Titration for ANA HEp-2BackgroundOften, only basic nuclear patterns, like homogenous, speckled, nucleolar, centromere or cytoplasmic, are reported by laboratories. The detection of other patterns requires well trained ...