Frey B. Amplification of genomic DNA by PCR. Reischl, U.: Molecular Diagnosis of Infectious Diseases. Totawa, New Jersey: Humana Press, 1998;143-156.Frey, B Amplification of genomic DNA by PCR In: Reischl, U (ed) 1998. Molecular Diagnosis of Infectious Diseases. Totawa, New Jersey: ...
Traditional methods of cloning a DNA sequence into a vector and replicating it in a living cell often require days or weeks of work, but amplification of DNA sequences by PCR requires only hours. While most biochemical analyses, including nucleic acid detection with radioisotopes, require the ...
REVI&SED UPDATED Edvo-Kit #330 330 PCR Amplification of DNA Experiment Objective: The objective of this experiment is for students to gain hands-on experience of the principles and practice of Polymerase Chain Reaction (PCR). The PCR products are then analyzed by agarose gel electrophoresis. ...
10X PCR buffer 10 μl Primer 1 1 μl Primer 2 1 μl dNTP 2 μl template DNA and water 85.5 μl Taq Polymerase 0.5 μl 2. Prepare a control reaction with no template DNA and an additional 10 μl of sterile water. 3. If the thermocycler does not have a heated lid, add 70-100...
MICROWAVE-ASSISTED PCR AMPLIFICATION OF DNA 优质文献 相似文献 参考文献Microwave Assisted PCR Amplification of DNA A method of microwave assisted nucleic acid amplification by PCR is disclosed. The method includes denaturing, annealing, and extending a nucleic acid sample, with at least the denaturing ...
One-armed PCR (OA-PCR) is a novel technique that allows amplification of genomic DNA from a single region of known sequence. Previously described methods are either limited to cDNA or require extensive manipulation of template prior to amplification. In OA-PCR, DNA from a small insert phage Fu...
PUF and PPR proteins are two families of 伪-helical repeat proteins that recognize single-stranded RNA sequences. Both protein families hold promise as scaffolds for designed RNA-binding domains. A modular protein RNA recognition code was apparent from the first crystal structures of a PUF protein...
First report of Cotton leaf curl Multan virus infecting Millettia pinnata in Pakistan (2003) Universal primers for the PCR-mediated amplification of DNA 1: a satellite-like molecule associated with begomovirus-DNA complexes. Keywords: ... MA Mahmood,RZ Naqvi,N Irfan,... - 《New Disease Reports...
We describe conditions that improve the specificity of amplification of a G + C-rich (57% G + C) DNA by PCR. Under standard conditions a 368-bp segment of the approx. 2.1-kb repeat unit of a satellite DNA that accounts for approx. 3% of the genome of the Bermuda land crab, Gecarci...
However, amplification of the correct sized fragments failed. Instead, PCR amplification pro- ducts ranging from 350 bp to several thousand base pairs were typ- ically observed (Fig. 1). Even the use of a non-proofreading but robust DNA polymerase, Taq (NE Biolabs) failed to amplify only ...