3.5 Amino Acid Composition Determination of the amino acid sequence of a purified protein begins with determination of the amino acid composition, i.e., the number of moles of each amino acid residue present per mole of protein, as follows. The protein is completely hydrolyzed by acid (6 N ...
Amino acidsarenatural compoundscomposed of amine (–NH2) andcarboxylic acid(–COOH) functional groups, linked to the same carbon atom (Figure 1.1). Different amino acids have a different and specific side chain linked to this exact carbon. There are more than 500 amino acids identified, some ...
Many of those MRPs are apparently produced through condensation reactions between the nucleophilic thiol residue and carbonyls as well as oxidative formation of disulphide links resulting in reaction products, which, in this way, cannot be produced by any other proteinogenic amino acid. Further ...
Branched-chain amino acid (BCAA) metabolism fulfills numerous physiological roles and can be harnessed to produce valuable chemicals. However, the lack of eukaryotic biosensors specific for BCAA-derived products has limited the ability to develop high-throughput screens for strain engineering and metaboli...
Two major proteolytic fragments of molecular masses 107 and 43 kDa originate as a result of a single cleavage in the vicinity of the 980th amino acid residue. Anti-β subunit monoclonal antibody PYN-2 inhibiting RNA polymerase activity at the stage of RNA elongation reacts with an epitope ...
The amino acid sequences of two cuttlefish protamine variants Sp1 and Sp2 have been established from automated sequence analysis and mass spectrometry data. Sp1 (57 residues) and Sp2 (56 residues) have molecular masses of 8410 and 8253 Da, respectively. They are almost identical proteins which ...
bulbs. Molecular masses of these inhibitors were found to be 7,370.2, 7,472.2, and 7,642.6 Da by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), respectively. Based on amino acid composition and N-terminal sequence, OTI-1 and -2 are the N-...
The BAN for barbitone or veronal (diethyl-malonyl-urea, 5,5-diethylbarbituric acid). Once widely used, as the sodium salt, as a sedative and hypnotic; also used in pH buffer solutions. barbiturates Class of drugs that depress activity of the central nervous system, largely superceded by ben...
The difference observed for the mutant Tyr661Ala in cleaving various substrates (Figure 2, Figure 3) suggests the differential function of this amino acid residue in substrate recognition under various conditions. However, the assay sensitivity and kinetics may also contribute to the variation observed...