The monolithic column is easy and quick to prepare, stable and uniform in structure, good in hydrophilic performance and has multiple amide functional groups. With the monolithic column, an on-column enrichment operation of glycopeptide based on the hydrophilic interaction mechanism in a microscale ...
Size-exclusion chromatography (SEC) assay. To probe effects on the aggregation state of NosA by the mutations, the size-exclusion chromatography assay was performed on a Superdex 75 column (10/300 GL) (GE Healthcare), which was previously equilibrated with buffer B (50 mM Tris-HCl,...
The organic layer was dried with MgSO4, the solvent removed under reduced pressure and the residue purified by column chromatography (SiOH, ethyl acetate/cyclohexane gradient). Synthesis of amides using ethoxyacetylene as activator. An oven-dried headspace vial with Teflon-coated stirring bar was ...
entry 1). Notably, both intramolecular carbene C–H insertion reaction (3’) and self-dimerization (3”) were almost suppressed. Further attempts to test different reaction temperatures revealed that 30 °C was the most efficient (Table1, entries 2...
This characteristic also is found for the alkyl–amide packing of this study, because the diisopropyl groups of the structure (Figure 1) afford the usual steric pro- tection against low-pH hydrolysis. Figure 3 shows that the rate of change for the alkyl–amide column when continuously purged ...
Metrizamide structure CAS No. 31112-62-6 Chemical Name: Metrizamide Synonyms amipaque;pyranose;WIN39103;WIN-39103;y-d-glucose;telebrix300;METRIZAMIDE;no)-2-deoxy-;Metrizamide (500 mg);METRIZAMIDE,ULTRAPURE CBNumber: CB8268280 Molecular Formula: C18H22I3N3O8 Molecular Weight: 789.1 ...
Carl Roth Silica gel 60, 0.04–0.063 mm was used for column chromatography. High-resolution mass spectra with electrospray ionization were recorded on a Bruker MicroOTOF II instrument (Bruker AXS, Karlsruhe, Germany). Melting points were determined by the capillary method on Electrothermal IA9000 ...
(3), from the column fractions of the crude ethyl acetate extract of the culture of a marine sponge-associated fungus,Aspergillus fischeriMMERU 23. The structure of4was elucidated by the interpretation of 1D and 2D NMR spectral data and high-resolution mass spectrum. The absolute configuration ...
The residue was purified by column chromatography and isolated intermediate 10 as an off white solid (606 mg; 94% yield). Step-2: Synthesis of 6-phenylnicotinic acid (Intermediate 11) To a solution Intermediate 10 (606 mg) in methanol (30 ml) was added 10% NaOH solution (2.5 ml) and...
5.6, which was chromatographed on a silica gel (25 g) column and eluted with CH2Cl2/MeOH v/v, 97:3 to yield 1 (2.8 mg). The enantiomeric mixture of 1 (2.2 mg) was applied on a chiral HPLC (90% n-hexane/isopropyl alcohol, flow rate 1 mL/min, Ultimate Cellu-D column 4.6 × ...