Pre-mRNA alternative splicing is a prevalent mechanism for diversifying eukaryotic transcriptomes and proteomes. Regulated alternative splicing plays a role in many biological processes, and dysregulated alternative splicing is a feature of many human di
3.8. Transcriptome-wide alternative splicing analysis in disease Aberrant splicing greatly contributes to the pathologies of many diseases as mentioned. Indeed, the detection of differential splicing not only serves as predictive, diagnostic, or prognostic biomarkers but also stratifies patients with differe...
analysis of NLRP3 splicing of exon 5, we have identified that NLRP3 splicing is likely not deterministic as we could identify cells expressing both the full-length NLRP3 isoform as well as the isoform lacking exon 5. Although this analysis cannot rule out that individual cells might be in ...
Diffuse large B-cell lymphoma (DLBCL) is the most common type of non-Hodgkin lymphoma, but the effect of AS on its pathogenesis has not been fully elucidated, so it is essential to find out DLBCL-specific splicing patterns and their potential as treatment targets.Material and Method RNA-Seq...
Alternative splicing (AS) plays a crucial role in transcriptomic diversity and is a hallmark of cancer that profoundly influences the development and progression of prostate cancer (PCa), a prevalent and potentially life-limiting cancer among men. Accumu
Nevertheless, little is known about the conservation of alternative splicing during fruit development among the fleshy fruits. Here, we carried out a comparative analysis of alterna- tive splicing during fruit development across cucumber, melon, papaya and peach spanning 123.2 million years [27] to ...
At the 5% FDR level, 38,146 pairs of genes out of ∼10 M examined pairs from GTEx lung tissue showed significant TE-splicing interaction effects, implying isoform-specific and/or sample-specific TE–TE correlations. Additional analysis across 13 GTEx brain tissues revealed strong tissue-...
We then explored the association between ancestry, sex, age, and BMI and AS variation by performing differential splicing analysis on the PSI value for each splicing event and correcting for known sources of technical variation and unobserved confounders such as cell-type composition (STAR Methods)....
Psoriasis is a chronic inflammatory disease that affects the skin, nails, and joints. For understanding the mechanism of psoriasis, though, alternative splicing analysis has received relatively little attention in the field. Here, we developed and applie
Alternative splicing is central to metazoan gene regulation, but the regulatory mechanisms are incompletely understood. Here, we show that G-quadruplex (G4) motifs are enriched ~3-fold near splice junctions. The importance of G4s in RNA is emphasised by