Replication complexMembraneAntiviralHost factorIn vitro RNA synthesisAlphaviruses are typically arthropod-borne, and many are important pathogens such as chikungunya virus. Alphaviruses encode four nonstructural proteins (nsP1-4), initially produced as a polyprotein P1234. nsP4 is the core RNA-...
NF-κB inhibition did not affect establishment of infection, replication complex formation, synthesis of nonstructural proteins or viral RNA synthesis in differentiated neurons. However, translation of structural proteins was impaired, eIF2α phosphorylation was decreased and host protein synthesis was ...
Alphavirus vectors can be used for vaccine delivery as naked RNA, DNA plasmids, or recombinant replication-deficient viral particles. While SIN and SFV have been studied in small animal models (Vajdy et al., 2001; Lemiale et al., 2001; Nilsson et al., 2001), VEE vector development has ...
Viruses are extremely complex and highly evolving microorganisms; thus, it is difficult to analyse them in detail. The virion is believed to contain all the essential components required from its entry to the establishment of a successful infection in a susceptible host cell. Hence, the virion com...
Positive-sense single-stranded RNA viruses, such as coronaviruses, flaviviruses and alphaviruses, carry out transcription and replication inside virus-induced membranous organelles within host cells1,2,3,4,5,6,7. The remodelling of the host-cell membranes for the formation of these organelles is ...
The alphavirus replication complex is found on the cytoplasmic surface of endosomes and lysosomes. We have shown that nsP1, one of the tour virus-encoded replicase proteins, is pahnitoylated and associated with these membranes when expressed alone in mammalian cells. Thus, nsPl may serve to ...
Development of Novel Small Molecule Inhibitors of Neurotropic Alphavirus Replication. 来自 ResearchGate 喜欢 0 阅读量: 28 作者: S Barraza 摘要: Emerging mosquito-borne viruses (arboviruses) constitute a large public health risk because of the ubiquity of mosquitoes and the potential for mosquito-...
1. A recombinant nucleic acid molecule comprising, in order: (i) a first nucleic acid sequence encoding a 5′ alphavirus replication recognition sequence, (ii) a second nucleic acid encoding alphavirus nonstructural proteins nsp1, nsp2, and nsp3; (iii) a nucleic acid selected from the group...
4) replication-defective adenovirus vector 复制缺陷型腺病毒载体 1. Objective: To investigate the role ofreplication-defective adenovirus vectorencoding human extracellular domain of vascular endothelial growth factor receptor-2 (hVEGFR-2 or hKDR) in induction of immunity against murine hepatocellular carci...
Alphaviruses are enveloped RNA viruses that contain several human pathogens. Due to intrinsic heterogeneity of alphavirus particles, a high resolution structure of the virion is currently lacking. Here we provide a 3.5 Å cryo-EM structure of Sindbi