Total sugar content and -amylase activity of normal seeds were higher than did aged seeds. The aged seeds with treatment of osmoconditioning and hardening increased total sugar content and --amylase activity, but hardening was more effective than osmoconditioning. The --amylase activity was ...
Alpha-amylase (EC 3.2.1.1) is a low-molecular-weight enzyme (approximately 45kDa) that cleaves the alpha-D-(1–4) glycan linkage of starch and glycogen. It has been in use as a diagnostic enzyme longer than any of the other enzymes. It is found in very high concentration in the pancr...
About thirty percent of two alpha-amylases produced from a serous papillary cystadenocarcinoma of the ovarium (case 1) and a bronchioloalveolar adenocarcinoma of the lung (case 2) was glycoproteins containing 1 mol of asparagine-linked sugar chain, respectively. The structures of the sugar moieties...
Medium temperature alpha-AmylaseDetail Mid-Temperature Alpha-Amylase is widely used in starch sugar, alcohol, brewery, textile, dye & printing and papermaking,etc. Working Mechanism: The enzyme hydrolyzes the α-1,4-glucosidic linkages of gelatinized starch randomly to soluble dextrins, reducing ...
alpha-Amylase from Bacillus amyloliquefaciens showed a distinct relationship between thermal stability and catalytic activity in the presence of different CaCl2 concentrations. The enzyme showed optimal activity in the presence of 1.0–2.0 mM CaCl2. The increase in CaCl2 concentration caused enzyme inhibitio...
We also describe the successful use of the CCSM method to improve the thermostability of α-amylase. Results and discussion α-Amylase is an important industrial biocatalyst in starch liquefaction processes and a valuable model enzyme for studies of thermal adaptation in proteins[29]. We used the ...
28° C. and can be irreversibly inactivated at temperatures above 28° C. The alpha-amylase of the present invention is dependent on calcium-ions, and activity is generally increased in the presence of detergents. In the following the enzyme of the present invention is referred to as AmyI3C...
18.The enzyme composition of claim 16, wherein the alpha-amylase at least 95% sequence identity to SEQ ID NO: 5. 19.The enzyme composition of claim 16 which is a starch hydrolyzing enzyme composition. 20.The enzyme composition of claim 16 which is a detergent composition. ...
In a pilot ex vivo study, a lentiviral vector was used to transfect patient hemopoietic stem cells to express α-galactosidase leading to increased enzyme activity [11]. Pre-clinical studies have investigated the use of zinc finger nuclease (ZFN) and adeno-associated viral (AAV) technologies to...
(Ray, 2004). Alpha-amylase fromA. oryzaewas the firstmicrobial enzymeto be manufactured and solid-state fermentation was initially used (Bogaret al., 2002; Rahardjoet al., 2005). To improve productivity, the manufacture of amylases now usessubmerged fermentationwithA. oryzaeandA. nigeras the ...