All_in_one_CRISPR-Cas9_LacZ质粒载体 价格￥680 起批量≥1支 最小起订1支 供货总量50支 发货地址浙江省温州市 建议售价￥680/支 更新日期2025年01月14日 产品规格0.5ug 即时洽谈立即询价查看联系方式 收藏产品发送留言 VIP3年 温州科淼生物科技有限公司 ...
The dual-plasmid CRISPR-Cas9 system suitable for iterative genome editing has been applied in Bacillus subtilis. However, it is limited by the selection of knockout genes, long editing cycle and instability. Results: To address these problems, we constructed an all-in-one plasmid CRISPR-Cas9 ...
#74293-20ulAll_in_one_CRISPR-Cas9_LacZ质粒20ul 立即咨询 +- 产品介绍 相关产品 》产品信息 货号名称规格别名 #74293All_in_one_CRISPR-Cas9_LacZ质粒 20ul 》产品描述 哺乳细胞基因编辑 》产品简介 Plasmid #74293 》产品图谱及序列 质粒来源addgene,由于数量众多,图谱序列上传速度慢,如有需要请联系客服人...
A selectable all-in-one CRISPR prime editing piggyBac transposon allows for highly efficient gene editing in human cell lines Reto Eggenschwiler, Thomas Gschwendtberger, Christian Felski, Christopher Jahn, Florian Langer, Jared Sterneckert, Andreas Hermann, Jonathan Lühmann, Doris Steinemann...
Furthermore, AAV-based systems are significantly more sensitive than other vectors (e.g., LVs) when it comes to packaging of multipart components, such as CRISPR/Cas24. As a consequence, most of the all-in-one CRISPR/Cas9 tools developed so far are based on plasmid-based or lentiviral del...
Each source node must have only one DNA molecule. Where PCR products are used in a source node, the primer sequences will automatically be modified by extension to provide the correct overlap sequence CRISPR digestion Perform CRISPR digestion by using selected sgRNA and substrates Table 2. ...
12.The nucleic acid molecule according to any one of claims 8-11, wherein the nucleic acid molecule is a plasmid. 13.The nucleic acid molecule according to claim 12, wherein the nucleic acid molecule is a single plasmid. 14.The nucleic acid molecule of any one of claims 8-13, wherein...
We believe interrogation of gene function can be performed in diverse cell lines in a rapid manner by using our all-in-one adenoviral CRISPR-Cas9 system since it does not involve conventional cloning, clonal selection and expansion.ResultsGeneration of all-in-one CRISPR-Cas9 vector using the ...
The CRISPR-Cas models used in DefenseFinder come from the macsy models of CasFinder availablehere. DefenseFinder website More information on defense systems on the DefenseFinder To make DefenseFinder results more intuitive, we created a Wiki of defense systems to gather information on all the def...
Manipulation of transcription factor expression has been used to programme cell fate and this approach has primarily involved the delivery of exogenous cDNA by plasmid or viral expression vectors. The CRISPR-CAS9 system has revolutionized genome editing1 but more recently the catalytically dead CAS9 (d...