#74293-20ulAll_in_one_CRISPR-Cas9_LacZ质粒20ul 立即咨询 +- 产品介绍 相关产品 》产品信息 货号名称规格别名 #74293All_in_one_CRISPR-Cas9_LacZ质粒 20ul 》产品描述 哺乳细胞基因编辑 》产品简介 Plasmid #74293 》产品图谱及序列 质粒来源addgene,由于数量众多,图谱序列上传速度慢,如有需要请联系客服人...
First, tolerance to potential pipetting errors regarding amounts of used plasmid DNA and LFS reagent was analyzed. To this end, a DNA range of 0.45–1.36 μg and a LFS range of 1.5–2.5 μl per well were examined. Measured PE efficiencies were largely indifferent in a wide range of both...
Gecko2人类全基因组CRISPR文库,All-in-one™ 一体化慢病毒颗粒(Gecko2载体); Synonyms: Gecko2 CRISPR Pool,Gecko2 Lenti Particles,Human CRISPR Pool at Sigma-Aldrich
CRISPR Cas9 表达系统 All-Cas9.pPuro说明书 Description:pAll-Cas9.pPuro is an all-in-one CRISPR/Cas expression system:1.The Cas9protein is expressed under the control of CMV promoter;2.The Cas9protein is an RNA-guided DNA endonuclease that cleaves the target DNA and generates site-directed ...
The dual-plasmid CRISPR-Cas9 system suitable for iterative genome editing has been applied in Bacillus subtilis. However, it is limited by the selection of knockout genes, long editing cycle and instability. Results: To address these problems, we constructed an all-in-one plasmid CRISPR-Cas9 ...
Bacillus subtilis, an important industrial microorganism, is commonly used in the production of industrial enzymes. Genome modification is often necessary to improve the production performance of cell. The dual-plasmid CRISPR-Cas9 system suitable for ite
Furthermore, AAV-based systems are significantly more sensitive than other vectors (e.g., LVs) when it comes to packaging of multipart components, such as CRISPR/Cas24. As a consequence, most of the all-in-one CRISPR/Cas9 tools developed so far are based on plasmid-based or lentiviral del...
We believe interrogation of gene function can be performed in diverse cell lines in a rapid manner by using our all-in-one adenoviral CRISPR-Cas9 system since it does not involve conventional cloning, clonal selection and expansion.ResultsGeneration of all-in-one CRISPR-Cas9 vector using the ...
Gecko2 Mouse Whole Genome CRISPR Pool, All-in-One™ Lenti Particles (Gecko2 vector); find Sigma-Aldrich-MGECKO2A MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich
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